| 引用本文: | 吴飞跃,林玳,顾卓珺,郑怀宇,傅军.α-倒捻子素脂质体的药动学研究[J].中国现代应用药学,2017,34(5):703-705. |
| WU Feiyue,LIN Dai,GU Zhuojun,ZHENG Huaiyu,FU Jun.Study on Pharmacokinetics of α-Mangostin Lipsome[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(5):703-705. |
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| 摘要: |
| 目的 采用LC-MS/MS考察α-倒捻子素脂质体的药动学特征。方法 采用SPF级ICR小鼠，♀，尾静脉注射α-倒捻子素脂质体3 mg·kg-1，用LC-MS/MS测定α-倒捻子素血药浓度，并计算其药动学参数。结果 α-倒捻子素线性范围为5~1 000 ng·mL-1（r=0.997 6），定量限为5 ng·mL-1，日内、日间精密度均<11.0%。主要药动学参数：t1/2为（0.99±0.12）h，Cmax为（1 524.60±323.82）ng·mL-1，AUC0-t为（645.84±63.74）ng·h·mL-1，MRT为（0.70±0.11）h，CL为（4.80±0.45）mL·h-1·kg-1，Vd为（6.80±1.00）mL·kg-1。结论 该法操作简便、快速、灵敏，可用于测定小鼠血浆中α-倒捻子素浓度。本研究结果可为药物的进一步研究提供依据。 |
| 关键词: α-倒捻子素 脂质体 液相色谱-质谱 药动学 |
| DOI：10.13748/j.cnki.issn1007-7693.2017.05.015 |
| 分类号: |
| 基金项目:舟山市医药卫生科技计划（2015G02） |
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| Study on Pharmacokinetics of α-Mangostin Lipsome |
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WU Feiyue1, LIN Dai1, GU Zhuojun1, ZHENG Huaiyu1, FU Jun2
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1.Department of Pharmacy, Zhoushan Hospital, Zhoushan 316000, China;2.Department of Pharmacy, the Tenth People's Hospital Affiliated to Tongji University, Shanghai 200072, China
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| Abstract: |
| OBJECTIVE To establish a sensitive and specific LC-MS/MS method for investigating the pharmacokinetics characteristic of α-mangostin liposome in mice. METHODS ICR mice were given in tail vein α-mangostin 3 mg·kg-1. The α-mangostin concentration was determined by LC-MS/MS and pharmacokinetic parameters was calculated. RESULTS The linearity ranged from 5 to 1 000 ng·mL-1 (r=0.997 6). The detectction of α-mangostin was 5 ng·mL-1. The RSDs within day and between days were <11%. The main pharmacokinetic parameters of t1/2, Cmax, AUC0-t, MRT, CL and Vss were (0.99±0.12)h, (1 524.60±323.82)ng·mL-1, (645.84±63.74)ng·h·mL-1, (0.70±0.11)h, (4.80±0.45)mL·h-1·kg-1, and (6.80±1.00)mL·kg-1, respectively. CONCLUSION The precision, accuracy and recovery of the method are found to be adequate for pharmacokinetic studies. The results can provide the basis for future research. |
| Key words: α-mangostin lipsome LC-MS/MS pharmacokinetics |
