| 引用本文: | 王斌,徐盈盈,林誉.HPLC波长切换联合梯度洗脱法同时测定消疲灵颗粒中的7种成分[J].中国现代应用药学,2017,34(4):571-574. |
| WANG Bin,XU Yingying,LIN Yu.Simultaneous Determination of Seven Components in Xiaopiling Granule by HPLC Wavelength Switching Combined Gradient Elution Method[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(4):571-574. |
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| 摘要: |
| 目的 建立同时测定消疲灵颗粒中7种成分含量的HPLC波长切换联合梯度洗脱方法。方法 采用Venusil MP-C18色谱柱，流动相为乙腈-1%冰醋酸溶液，流速为0.9 mL·min-1，梯度洗脱，柱温为30 ℃，进样量为10 μL。结果 牡荆素葡萄糖苷、牡荆素鼠李糖苷、牡荆素、金丝桃苷、芒柄花苷、毛蕊异黄酮和芒柄花素检测浓度分别在2.56~51.20 μg·mL-1，14.87~297.40 μg·mL-1，2.14~42.80 μg·mL-1，3.16~63.20 μg·mL-1，3.80~76.00 μg·mL-1，2.14~42.80 μg·mL-1，4.81~ 96.20 μg·mL-1内与峰面积呈良好的线性关系（r≥0.999 1），平均回收率97.0%~100.0%，RSD 0.55%~1.67%，精密度和重复性良好，供试品溶液在室温条件下12 h内稳定。结论 该方法操作简便，精密度、稳定性、重复性好，可用于消疲灵颗粒中7种有效成分含量的同时测定。 |
| 关键词: 消疲灵颗粒 牡荆素葡萄糖苷 牡荆素鼠李糖苷 牡荆素 金丝桃苷 芒柄花苷 毛蕊异黄酮 芒柄花素 波长切换联合梯度洗脱法 中成药多组分测定 |
| DOI：10.13748/j.cnki.issn1007-7693.2017.04.021 |
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| Simultaneous Determination of Seven Components in Xiaopiling Granule by HPLC Wavelength Switching Combined Gradient Elution Method |
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WANG Bin, XU Yingying, LIN Yu
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Department of Pharmacy, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China
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| Abstract: |
| OBJECTIVE To develop an HPLC wavelength switching combined gradient elution method for determination of seven components in Xiaopiling granule simultaneously. METHODS The determination was performed on Venusil MP-C18 column with mobile phase consisted of acetonitrile(A)-1% glacial acetic acid solution (B) at the flow rate of 0.9 mL·min-1, gradient elution and the injection volum was 10 μL. The column temperature was 30 ℃. RESULTS The linear ranges of vitexin-4”-O-glucoside, vitexin-2”-o-rhamnoside, vitexin, hyperoside, ononin, calycosin and formononetin were 2.56- 51.20 μg·mL-1, 14.87-297.40 μg·mL-1, 2.14-42.80 μg·mL-1, 3.16-63.20 μg·mL-1, 3.80-76.00 μg·mL-1, 2.14-42.80 μg·mL-1, 4.81-96.20 μg·mL-1, respectively(r≥0.999 1); the average recoveries were 97.0%-100.0% and the corresponding RSD was 0.55%-1.67%, respectively. The precision and the repeatability were good. Test solution was stable at room temperature in 12 h. CONCLUSION The method is simple with good precision, stability and reproducibility, and it can be used for the simultaneous determination of 7 components in Xiaopiling granule. |
| Key words: Xiaopiling granule vitexin-4”-O-glucoside vitexin-2”-O-rhamnoside vitexin hyperoside ononin calycosin formononetin wavelength switching combined gradient elution method multicomponent determination of chinese patent drug |
