7-二氟甲氧基-5,4'-二甲氧基金雀异黄素上调miR-26b对妊娠期糖尿病脂肪细胞葡萄糖摄取的影响

孟娅妮; 于海伦

引用本文:	孟娅妮,于海伦.7-二氟甲氧基-5,4'-二甲氧基金雀异黄素上调miR-26b对妊娠期糖尿病脂肪细胞葡萄糖摄取的影响[J].中国现代应用药学,2017,34(8):1104-1108.
	MENG Yani,YU Hailun.Effects of 7-Difluoromethoxy-5,4'-dimethoxygenistein on Adipocyte Cells of Gestational Diabetes Mellitus In-taking Glucose via Up-regulation miR-26b[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(8):1104-1108.

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7-二氟甲氧基-5,4'-二甲氧基金雀异黄素上调miR-26b对妊娠期糖尿病脂肪细胞葡萄糖摄取的影响
孟娅妮¹, 于海伦^1,2
1.杭州市妇产科医院妇产科, 杭州 310008;2.山东省淄博市临淄区人民医院产科, 山东淄博 255400

摘要:

目的探究7-二氟甲氧基-5，4'-二甲氧基金雀异黄素（7-difluoromethoxy-5，4'-dimethoxygenistein，DFMG）对妊娠期糖尿病（gestational diabetes mellitus，GDM）肠系膜脂肪细胞摄取葡萄糖的影响。方法提取GDM孕妇肠系膜脂肪细胞，MTT检测不同浓度DFMG对脂肪细胞的毒性；液体闪烁仪检测脂肪细胞对[³H]-2-脱氧-D-葡萄糖的摄取率；RT-PCR检测孕妇肠系膜脂肪细胞miR-26b及其下游靶基因PTEN mRNA表达变化；Western blotting检测IRS-PI3K/AKT-mGLUT4信号通路相关蛋白的表达。结果 DFMG对体外培养GDM孕妇肠系膜脂肪细胞无毒副作用，与GDM组相比，液体闪烁仪提示DFMG可显著促进GDM肠系膜脂肪细胞对[³H]-2-脱氧-D-葡萄糖的摄取率（P<0.05）。RT-PCR提示DFMG可以显著上调GDM脂肪细胞miR-26b表达（P<0.05），下调PTEN mRNA表达（P<0.05）。Western blotting提示DFMG可以下调PTEN蛋白表达（P<0.05），上调p-PI3K、p-AKT和mGLUT4蛋白表达（P<0.05）。结论 DFMG可能通过上调miR-26b表达，调节IRS-PI3K/AKT-GLUT4信号通路，调节GDM肠系膜脂肪细胞对葡萄糖的摄取率。

关键词: 7-二氟甲氧基-5,4'-二甲氧基金雀异黄素 miR-26b 妊娠期糖尿病

DOI：10.13748/j.cnki.issn1007-7693.2017.08.007

分类号:R965.2

基金项目:

Effects of 7-Difluoromethoxy-5,4'-dimethoxygenistein on Adipocyte Cells of Gestational Diabetes Mellitus In-taking Glucose via Up-regulation miR-26b

MENG Yani¹, YU Hailun^1,2

1.Deparetment of Obstetrics and Gynecolgy, Hangzhou Obstetrics and Gynecolgy Hospital, Hangzhou 310008, China;2.Deparetment of Obstetrics, Zibo Linzi Distict People's Hospital, Zibo 255400, China

Abstract:

OBJECTIVE To investigate the effects of 7-difluoromethoxy-5,4'-dimethoxygenistein(DFMG) on adipocyte cells of gestational diabetes mellitus(GDM) in-taking glucose. METHODS Adipocyte cells were extracted from mesenteric adipose tissue of GDM patients. The toxicity of different concentrations of DFMG for adipocyte cells were determined by MTT assay. The rate of adipocyte cells in-taking[³H]-2-decoxy-glucose was evaluated by liquid scintillation detector. The expression of miR-26b and its downstream target gene PTEN mRNA were examined by RT-PCR assay. The proteins expression related to IRS-PI3K/AKT-mGLUT4 signaling pathways were assessed using Western blotting method. RESULTS There was no toxide side effect of DFMG on akipocyte cells of GDM in vitro. Liquid scintillation detector showed DFMG could prompt the rate of adipocyte cells in-taking[³H]-2-decoxy-glucosecompared with GDM group(P<0.05). RT-PCR method indicated DFMG could up-regulate the expression of miR-26b and down-regulate its target gene PTEN mRNA compared with GDM group(P<0.05). Western blotting assay showed DFMG could reduce the expression of PTEN protein compared with GDM group(P<0.05), enhance the expressions of p-PI3K, p-AKT and mGLUT4 proteins(P<0.05). CONCLUSION DFMG can promote the rate of adipocyte cells in-taking[³H]-2-decoxy-glucose via up-regulating miR-26b expression, adjusting the IRS-PI3K/AKT-GLUT4 signaling pathways.

Key words: 7-difluoromethoxy-5,4'-dimethoxygenistein miR-26b gestational diabetes mellitus