| 引用本文: | 罗慧英,曹茸茸,黄亚红.藏药蕨麻对原代培养小鼠肝细胞酒精损伤保护作用研究[J].中国现代应用药学,2016,33(9):1130-1133. |
| LUO Huiying,CAO Rongrong,HUANG Yahong.Protective Effect of Tibetan Medicine Potentilla Anserina L. on Ethanol-induced Damage in Prime Liver Cell of Mice[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(9):1130-1133. |
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| 摘要: |
| 目的 研究藏药蕨麻对原代培养酒精损伤肝细胞的保护作用。方法 原代肝细胞经分离纯化培养后，MTT法评价藏药蕨麻对酒精损伤肝细胞存活率的影响；荧光染色法测定藏药蕨麻对酒精损伤肝细胞活性氧物质(ROS)含量、细胞内钙离子浓度的影响；流式细胞仪检测藏药蕨麻对酒精损伤肝细胞凋亡的影响；免疫印迹法检测藏药蕨麻对Bcl-2和Bax表达的影响。结果 经酒精损伤后，肝细胞存活率降低；细胞内ROS含量和钙离子浓度增高；凋亡抑制基因Bcl-2减弱、促凋亡基因Bax表达增强。藏药蕨麻可明显提高细胞存活率；降低细胞内ROS含量和钙离子浓度；改善凋亡情况，增强Bcl-2表达，抑制Bax表达，且作用呈剂量依赖性。结论 藏药蕨麻对原代培养小鼠肝细胞酒精损伤具有一定的保护作用。 |
| 关键词: 藏药蕨麻 原代培养肝细胞 酒精性肝损伤 |
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| 基金项目:甘肃省教育厅高校科研项目(2013A-078) |
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| Protective Effect of Tibetan Medicine Potentilla Anserina L. on Ethanol-induced Damage in Prime Liver Cell of Mice |
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LUO Huiying1,2, CAO Rongrong1, HUANG Yahong1
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1.Department of Pharmacology, Gansu University of Chinese Medicine, Lanzhou 730000, China;2.Key Laboratory of Pharmacology and Toxicology for Traditional Chinese Medicines of Gansu Province, Lanzhou 730000, China
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| Abstract: |
| OBJECTIVE To study the protective effct of Tibetan medicine Potentilla anserina L. on ethanol-induced damage in mice prime liver cell. METHODS After isolation, purification and culture of primary hepatocytes, the livability of cell was evaluated by MTT; fluorescence microscopy were used to evaluated the contents of reactive oxygen species (ROS) and concentration of Ca2+; flow cytometry was used to detect the cell apoptosis; the expression of Bcl-2 and Bax were detected by Western bloting. RESULTS After damaged by alcohol, the cell livability decreased, the contents of Ros and concentration of Ca2+ increased, the expression of Bcl-2 decreased while that of Bax increased. Potentilla anserina could increase the cell livability, decreased the intracellular ROS content and Ca2+ concentration ameliorate the apoptosis, increase the expression of Bcl-2, and decrease the expression of Bax. CONCLUSION Tibetan medicine Potentilla Anserina L. has protective effect on ethanol-induced damage in prime liver cell of mice. |
| Key words: Potentilla anserine L. prime hepatic cell ethanol-induced liver damage |
