| 引用本文: | 邓霖芳,谭蓓蓓,窦冕,郝丽莉,刘江云,杨世林.HPLC同时测定猫豆中左旋多巴及其衍生物含量[J].中国现代应用药学,2016,33(7):845-848. |
| DENG Linfang,TAN Beibei,DOU Mian,HAO Lili,LIU Jiangyun,YANG Shilin.Simultaneous Determination of L-dopa and Its Derivates in Seeds of Mucuna Pruriens Var. Utilis by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(7):845-848. |
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| HPLC同时测定猫豆中左旋多巴及其衍生物含量 |
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邓霖芳1, 谭蓓蓓1, 窦冕1,2, 郝丽莉1, 刘江云1, 杨世林1
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1.苏州大学医学部药学院，苏州 215123;2.滨州医学院附属医院，山东 滨州 256603
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| 摘要: |
| 目的 建立同时测定猫豆中左旋多巴(2)及其3种衍生物含量的高效液相色谱法。方法 从猫豆中分离获得3-羧基-6,7-二羟基-1,2,3,4-四氢异喹啉(1)、1-甲基-3-羧基-6,7-二羟基-1,2,3,4-四氢异喹啉(3)、1,1二甲基-3-羧基-6,7-二羟基-1,2,3,4-四氢异喹啉(4)等3种成分；含量测定采用Cosmosil C18-PAQ色谱柱(4.6 mm×250 mm，5 mm)，流动相1%乙酸等度洗脱，流速0.5 mL·min-1，柱温30 ℃，检测波长280 nm。结果 左旋多巴(2)和3个化合物色谱分离良好，分别在0.298 4~5.968 mg (1)，6.554~131.1 mg (2)，0.262 6~5.252 mg (3)，0.256 8~5.136 mg (4)线性关系良好(r>0.999)，平均加样回收率依次为97.7%，99.1%，98.7%，97.9%。结论 本方法简便、准确，可用于同时测定猫豆中的左旋多巴及其四氢异喹啉类衍生物含量，为其质量控制方法提供参考。 |
| 关键词: 猫豆 左旋多巴 四氢异喹啉 高效液相色谱法 |
| DOI： |
| 分类号:R284.1；R917.101 |
| 基金项目:国家自然科学基金项目(81274190) |
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| Simultaneous Determination of L-dopa and Its Derivates in Seeds of Mucuna Pruriens Var. Utilis by HPLC |
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DENG Linfang1, TAN Beibei1, DOU Mian1,2, HAO Lili1, LIU Jiangyun1, YANG Shilin1
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1.College of Pharmaceutical Sciences, SooChow University, Suzhou 215123, China;2.The Affiliated Hospital of Binzhou Medical University, Binzhou 256603, China
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| Abstract: |
| OBJECTIVE To establish a method for simultaneous determination of both L-dopa(2) and its derivates in seeds of Mucuna pruriens var. utilis Burck by HPLC. METHODS Three ingredients, 3-carboxy-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline(1), 1-methyl-3-carboxy-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline(3) and 1,1-dimethyl-3-carboxy- 6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline(4) were isolated from the herb. Chromatographic separation was performed on a Cosmosil C18-PAQ analytical column (4.6 mm×250 mm, 5 mm) with 1% acetic acid as the mobile phase at 30 ℃. The detection wavelength was 280 nm and the flow rate was 0.5 mL·min-1. RESULTS The 4 components were separated with satisfaction. Their linearity ranges were between 0.298 4-5.968 mg (1), 6.554-131.1 mg (2, L-dopa), 0.262 6-5.252 mg (3) and 0.256 8-5.136 mg (4), respectively with their correlation coefficients all >0.999. Their average recovery rates were 97.7%, 99.1%, 98.7% and 97.9%. CONCLUSION The method is accurate and selective for simultaneous determination of 4 ingredients in seeds of Mucuna pruriens, which could be applied for the quality control of this herb. |
| Key words: Mucuna pruriens var. utilis L-dopa tetrahydroisoquinoline HPLC |
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