| 引用本文: | 孙小明,裴栋,刘晔玮,邸多隆.辅料对油橄榄叶提取物中橄榄苦苷与羟基酪醇稳定性的影响[J].中国现代应用药学,2016,33(3):261-265. |
| SUN Xiaoming,PEI Dong,LIU Yewei,DI Duolong.Impact of the Excipients on the Stabilities of Oleuropein and Hydroxytyrosol in Olive Leaf Extract[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(3):261-265. |
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| 摘要: |
| 目的 考察辅料种类、温度和时间对油橄榄叶中橄榄苦苷和羟基酪醇稳定性和均一性的影响。方法 橄榄苦苷与羟基酪醇的检测采用SinoChrom ODS-BP色谱柱(4.6 mm×250 mm，5 μm)，以乙腈-水(25∶75)为流动相，流速1.0 mL·min-1，检测波长232 nm。结果 以淀粉、羧甲基淀粉钠、微晶纤维素、低取代羟丙纤维素和滑石粉为辅料制得颗粒中的橄榄苦苷与羟基酪醇含量稳定。制颗粒过程中采用70 ℃烘干1 h即可。稠浸膏于4 ℃条件下，密闭遮光放置96 h后，其橄榄苦苷与羟基酪醇含量均一且稳定。结论 上述制剂辅料可用于油橄榄叶提取物的颗粒剂或片剂研究，且含量均一、稳定。 |
| 关键词: 油橄榄叶 橄榄苦苷 羟基酪醇 均一性 稳定性 高效液相色谱法 辅料 |
| DOI： |
| 分类号:R284.1；R917.101 |
| 基金项目:国家自然科学基金(21205126)；国家高技术研究发展计划(863计划，2014AA022203) |
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| Impact of the Excipients on the Stabilities of Oleuropein and Hydroxytyrosol in Olive Leaf Extract |
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SUN Xiaoming1, PEI Dong1, LIU Yewei2, DI Duolong1
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1.Key Laboratory for Natural Medicine of Gansu Province, Key Laboratory of Chemistry of Northwestern Plant Resources, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China;2.Institute of Nutrition and Food Hygiene of Public Health, Lanzhou University, Lanzhou 730000, China
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| Abstract: |
| OBJECTIVE To investigate the influence of excipients, temperature and time on the stability and uniformity of hydroxytyrosol and oleuropein in oleave leaf extract. METHODS The contents of hydroxytyrosol and oleuropein were analyzed by high performance liquid chromatography, equipped with SinoChrom ODS-BP column(4.6 mm×250 mm, 5 μm). The mobile phases contain acetonitrile and water (25∶75). The flow rate was 1.0 mL·min-1 and detection wavelength was 232?nm. RESULTS The contents of hydroxytyrosol and oleuropein in solid particles contained starch, sodium carboxymethyl starch, microcrystalline cellulose, low substituted hydroxypropy cellulose and talcum powder were stable. During the course of preparation, the best condition of 70 ℃ and 1 h should be chosen. Besides, under 4 ℃ and 96 h, the contents of hydroxytyrosol and oleuropein were still stable and uniform. CONCLUSION The above excipients can be used in the pharmaceutics studies and the main compounds in extract are stable. |
| Key words: olive leaves oleuropein hydroxytyrosol homogeneity stability HPLC excipients |
