| 引用本文: | 陈为飞,李渊贞,朱希,王玲,尹华.HPLC同时测定补肾活血颗粒中松脂醇二葡萄糖苷、苦杏仁苷、桂皮醛的含量[J].中国现代应用药学,2015,32(4):463-466. |
| CHEN Weifei,LI Yuanzhen,ZHU Xi,WANG Ling,YIN Hua.Simultaneous Determination of Pinoresinol Diglucoside, Amygdalin and Cinnamylaldehyde in Bushen Huoxue Granule[J].Chin J Mod Appl Pharm(中国现代应用药学),2015,32(4):463-466. |
|
| 摘要: |
| 目的 采用HPLC波长切换法，建立同时测定补肾活血颗粒中松脂醇二葡萄糖苷、苦杏仁苷、桂皮醛3成分含量的分析方法。方法 采用Zorbax Extend-C18柱(4.6 mm×150 mm，5 μm)；以乙腈-水为流动相梯度洗脱，松脂醇二葡萄糖苷、苦杏仁苷、桂皮醛的检测波长分别为227，210，290 nm；进行样品前处理工艺和方法学考察。结果 松脂醇二葡萄糖苷、苦杏仁苷和桂皮醛均得到很好分离，浓度分别在0.426 0~2.88 6 μg?mL-1，7.455~86.60 μg?mL-1和0.440 6~13.22 μg?mL-1内线性关系良好(r≥0.999)；平均加样回收率分别为98.3%(RSD=1.6%)，101.4%(RSD=1.1%)和100.0%(RSD=1.2%)；精密度试验峰面积RSD均≤1.4%。结论 该方法预处理简单，方法灵敏、准确、重复性好，可用于补肾活血颗粒及相关制剂的质量控制。 |
| 关键词: 补肾活血颗粒 高效液相色谱法 波长切换法 含量测定 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金项目(81273772)；浙江省中医药科学研究基金计划项目(2010ZA026)；浙江省中药现代化项目(浙经信[2010]421号) |
|
| Simultaneous Determination of Pinoresinol Diglucoside, Amygdalin and Cinnamylaldehyde in Bushen Huoxue Granule |
|
CHEN Weifei1, LI Yuanzhen2, ZHU Xi2, WANG Ling2, YIN Hua2
|
|
1.Zhejiang Starry Pharmaceutical Co., Ltd., Xianju 317300, China;2.College of Pharmacy, Zhejiang Chinese Medicine University, Hangzhou 310053, China
|
| Abstract: |
| OBJECTIVE To establish an HPLC method for determination of pinoresinol diglucoside, amygdalin, and cinnamylaldehyde in Bushen Huoxue granule. METHODS Agilent Zorbax Extend-C18(4.6 mm×150 mm, 5 μm) was adopted; the mobile phase was acetonitrile-water with gradient elution at a flow rate of 1.0 mL?min-1, and the detection wavelength was 210 nm(0-5 min) for amygdalin, 227 nm(5?10 min) for pinoresinol diglucoside, 290 nm(10-14 min) for cinnamylaldehyde. The methodological study included sample pre-treatment process and method validation. RESULTS The components of pinoresinol diglucoside, amygdalin, and cinnamylaldehyde were well separated, the method had a good linearity in the range of 0.426 0-2.88 6 μg?mL-1 7.455-86.60 μg?mL-1and 0.440 6?13.22 μg?mL-1(r≥0.999); the average recoveries were 98.3% (RSD=1.6%), 101.4%(RSD=1.1%), 100.0%(RSD=1.2%), respectively, and the RSDs of intra-day and inter-day assays were all ≤1.4%. CONCLUSION This method was simple, sensitive and reproducible and can be used for the quality control of Bushen Huoxue granule and its related preparation. |
| Key words: Bushen Huoxue granule HPLC wavelength switching content determination |
