| 引用本文: | 浦香兰,张云天.茵陈配方颗粒HPLC特征图谱研究[J].中国现代应用药学,2014,31(10):1195-1197. |
| PU Xianglan,ZHANG Yuntian.HPLC Characteristic Chromatograms of Formula Granule of Artemisiae Scopariae Herba[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(10):1195-1197. |
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| 摘要: |
| 目的 建立茵陈配方颗粒高效液相色谱(HPLC)特征图谱。方法 色谱柱为Megres5-C18 (250 mm×4.6 mm，5 μm)，流动相为乙腈-0.1%磷酸水溶液，梯度洗脱，流速为 1.0 mL·min-1，检测波长为 327 nm，柱温为 30 ℃，进样量为 10 μL。结果 由 8 个共有峰构成茵陈配方颗粒的特征图谱，11批样品的图谱与对照图谱的相似度为 0.963~0.998，可以用于茵陈配方颗粒的定性鉴别。结论 该法精密度、稳定性、重复性良好，可为茵陈配方颗粒的鉴别和质量评价提供参考。 |
| 关键词: 茵陈配方颗粒 特征图谱 高效液相色谱法 |
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| 基金项目:上海市中医药标准化研究项目(ZYSNXD-CC-BZH) |
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| HPLC Characteristic Chromatograms of Formula Granule of Artemisiae Scopariae Herba |
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PU Xianglan, ZHANG Yuntian
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Jiangyin Tianjiang Pharmaceutical Co., Ltd, Jiangyin 214434, China
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| Abstract: |
| OBJECTIVE To establish the HPLC characteristic chromatogram of Formula Granule of Artemisiae Scopariae Herba. METHODS The chromatograms were obtained using Megres5-C18 (250 mm×4.6 mm, 5 μm) with mobile phase consisted of acetonitrile and 0.1% phosphoric acid solution in gradient elution at flow rate of 1.0 mL·min-1, detection wavelength of 327 nm, column temperature of 30 ℃ and sample volume of 10 μL. RESULTS The HPLC characteristic chromatogram of Formula of Artemisiae Scopariae Herba consisted of 8 common peaks. The similarity of 11 batches of samples was within 0.963-0.998. CONCLUSION The HPLC method is proved to be accurate, stable and reproducible, and it can provide reference for the identification and quality control of Formula Granule of Artemisiae Scopariae Herba. |
| Key words: Formula Granule of Artemisiae Scopariae Herba characteristic chromatogram HPLC |
