LKB-1表达差异细胞系中黄芩苷和顺铂联合用药研究

王士群; 庞丽萍; 朱宇珍; 叶华; 郑学宝<sup>*</sup>

引用本文:	王士群,庞丽萍,朱宇珍,叶华,郑学宝.LKB-1表达差异细胞系中黄芩苷和顺铂联合用药研究[J].中国现代应用药学,2014,31(8):907-911.
	WANG Shiqun,PANG Liping,ZHU Yuzhen,YE Hua,ZHENG Xuebao.Study on the Drug Combination of Cisplatin and Baicalin in LKB-1 Differential Expression Cell Lines[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(8):907-911.

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LKB-1表达差异细胞系中黄芩苷和顺铂联合用药研究
王士群^1,2, 庞丽萍³, 朱宇珍², 叶华², 郑学宝²
1.广东医学院药理学教研室，广东湛江 524023;2.广东天然药物研究与开发重点实验室，广东湛江 524023;3.广东医学院附属医院消化内科，广东湛江 524023

摘要:

黄芩苷是黄芩中提取的黄酮类天然成分，具有显著的抗炎、抗肿瘤、促代谢等生理活性。腺苷酸活化蛋白激酶(AMPK)是调控能源代谢的主要途径，由上游LKB-1或CaMKKβ蛋白激酶调控。最新研究发现黄芩苷对于LKB-1低表达的细胞系中可通过CaMKKβ途径激活AMPK。本研究通过等效线图解法和Chou-Talalay法定性及定量评估了黄芩苷和顺铂在LKB-1表达差异的细胞系的相互作用。细胞凋亡结果发现：相比LKB-1高表达的肿瘤细胞系HepG2，黄芩苷在LKB-1低表达细胞系A549能够增强顺铂对凋亡的诱导；相反，对于高表达LKB-1的正常细胞系LO2，黄芩苷起到保护作用。

关键词: 黄芩苷顺铂 LKB-1 等效线图解法 Chou-Talalay

DOI：

分类号:

基金项目:国家自然科学基金面上项目(81173240)

Study on the Drug Combination of Cisplatin and Baicalin in LKB-1 Differential Expression Cell Lines

WANG Shiqun^1,2, PANG Liping³, ZHU Yuzhen², YE Hua², ZHENG Xuebao²

1.Department of Pharmacology, Guangdong Medical College, Zhanjiang 524023, China;2.Guangdong Key Laboratory for Research and Development of Natural Drugs, Zhanjiang 524023, China;3.Department of Gastroenterology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524023, China

Abstract:

Baicalin, one of the major flavoniods in Scutellaria baicalensis, is a potent activity in anti-inflammatory, anti-tumor and promoting metabolism. AMP-activated protein kinase(AMPK) plays an important role in energy metabolism and regulated mainly by two upstream kinases, LKB-1 or CaMKK. Recent studies show that CaMKKβ is involved in AMPK by baicalin in LKB-1 deficient cell lines. In this study, isobologram and Chou-Talalay was respectively applied to qualitative and quantitative assessment of the interaction of cisplatin and baicalin in LKB-1 deficient or sufficient cell lines. In the apoptosis results, it was found that compared with LKB-1 sufficient tumor cell lines, baicalin could enhance the rate of apoptosis induced by cisplatin in deficient cells. On the contrary, baicalin protected against the development of apoptosis in LO2 cells, a LKB-1 sufficient liver cell lines.

Key words: baicalin cisplatin LKB-1 isobologram Chou-Talalay