| 引用本文: | 张昀,刘路.补骨脂酚在大鼠血清、肝和肾中的含量测定[J].中国现代应用药学,2014,31(8):984-987. |
| ZHANG Yun,LIU Lu.Determination of Bakuchiol in Serum, Liver and Kidney of Rats[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(8):984-987. |
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| 摘要: |
| 目的 建立一种快速检测大鼠灌胃给药后，补骨脂酚在血清、肝脏、肾脏中含量测定的方法。方法 Agilent HC-C18柱(4.6 mm×250 mm，5 mm)；流动相：0.1%甲酸水溶液(A)-乙腈(B)，梯度洗脱(0~10 min，B 35%→45%，10~25 min，B 80%)，流速：1 mL·min?1，柱温：30 ℃，检测波长：0~10 min，295 nm(氯霉素)，10~25 min，260 nm(补骨脂酚)。结果 补骨脂酚在血清、肝匀浆液、肾匀浆液中均能达到很好的分离，在0.1~10.0 mg·L?1内有良好的线性关系。结论 本方法简便准确，可用于测定给药后血清、肝脏、肾脏中补骨脂酚的含量。 |
| 关键词: 补骨脂 补骨脂酚 高效液相色谱法 |
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| 基金项目:河南大学民生学院教育教学改革研究项目资助计划(MSJG2013-41)；河南省教育厅研究项目(14B350009) |
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| Determination of Bakuchiol in Serum, Liver and Kidney of Rats |
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ZHANG Yun1, LIU Lu2
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1.Minsheng College, Pharmacy College, Henan University, Kaifeng 475004, China;2.Pharmacy College, Henan University, Kaifeng 475004, China
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| Abstract: |
| OBJECTIVE To establish a quantitaive determination method of bakuchiol in serum, liver and kidney of rats by HPLC. METHODS The HPLC method was used with the Agilent HC-C18 and the mobile phase was 0.1% formic acid-acetonitrile (65∶35) [0-10 min, B 35%→45%, 10-25 min, B 80%] in the flow rate of 1 mL·min?1. The detection wavelength was set at 295 nm(chloramphenicol) in 0-10 min and 260 nm(bakuchiol) in 10-25 min with column temperature of 30 ℃. RESULTS The bakuchiol had a good linear between 0.1-10.0 mg·L?1 in serum, liver and kidney of rats. CONCLUSION This method can determine the content of bakuchiol in serum, liver and kidney of rats. |
| Key words: psoralen bakuchiol HPLC |
