| 引用本文: | 吴健,高家荣,韩燕全,李翔.UPLC同时测定复方守宫散中核苷类含量[J].中国现代应用药学,2014,31(7):843-846. |
| WU Jian,GAO Jiarong,HAN Yanquan,LI Xiang.Simultaneous Determination of Nucleoside Contents in Compound Shougong Powder by UPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(7):843-846. |
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| 摘要: |
| 目的 建立UPLC同时测定复方守宫散中腺嘌呤、腺苷含量的方法。方法 色谱柱Acquity BEH C18(2.1 mm× 100 mm，1.7 μm)，甲醇-水为流动相，梯度洗脱，流速：0.25 mL·min-1，柱温：26 ℃，检测波长：254 nm。结果 腺嘌呤、腺苷的进样量分别在0.040 9~0.511 5 mg·mL-1(r=0.999 9)和0.057 8~0.722 0 mg·mL-1(r=0.999 6)内与其峰面积呈良好的线性关系；平均回收率分别为97.7%，97.7%，RSD分别为0.96%，1.18%。结论 该方法快速、可靠、准确，可作为复方守宫散的质量控制方法。 |
| 关键词: 超高效液相色谱 复方守宫散 腺嘌呤 腺苷 含量测定 |
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| 基金项目:国家中医药重点学科(临床中药学)建设项目(国中医药人教发[2012]32号)；安徽中医药大学青年科学研究基金项目(2013qn019) |
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| Simultaneous Determination of Nucleoside Contents in Compound Shougong Powder by UPLC |
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WU Jian1,2, GAO Jiarong1,2, HAN Yanquan1, LI Xiang1
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1.The First Affiliated Hospital of Anhui University of Chinese Medicine, Grade 3 Laboratory of TCM Preparation, State Administration of TCM, Hefei 230031, China;2.Anhui University of Chinese Medicine, Hefei 230032, China
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| Abstract: |
| OBJECTIVE To establish a UPLC method for the determination of adenine and adenosine in Compound Shougong powder. METHODS The determination was performed on Acquity BEH C18(2.1 mm×100 mm, 1.7 μm) with mobile phase consisted of methabol-water(gradient elution) at a flow rate of 0.25 mL·min-1. Column temperature was 26 ℃. The UV detection wavelength was set at 254 nm. RESULTS The calibration curves were linear in the range of 0.040 9-0.511 5 mg·mL-1(r=0.999 9) for adenine, 0.057 8-0.722 0 mg·mL-1(r=0.999 6) for adenosine. The average recoveries were 97.7%, 97.7% and RSDs were 0.96%, 1.18%, respectively. CONCLUSION The determination method is fast, reliable, accurate, and can be used for quality control of Compound Shougong powder. |
| Key words: UPLC Compound Shougong powder adenine adenosine content determination |
