| 引用本文: | 谢元勋,何厚洪,盛卫国,叶剑锋,吴健,王如伟.HPLC-ELSD测定油菜蜂花粉中6种脂肪酸[J].中国现代应用药学,2014,31(5):559-563. |
| XIE Yuanxun,HE Houhong,SHENG Weiguo,YE Jianfeng,WU Jian,WANG Ruwei.Determination of Six Kinds of Fatty Acids in Rape Bee Pollen by HPLC-ELSD[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(5):559-563. |
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| 摘要: |
| 目的 建立HPLC-ELSD法分析油菜蜂花粉中6种脂肪酸，并测定其含量。方法 色谱柱Agilent ZORBAX SB-C18(4.6 mm×250 mm，5 μm)；流动相：甲醇-1%乙酸水梯度洗脱，流速1.2 mL·min-1，柱温25 ℃；检测器参数：增益：6.0，漂移管温度：30 ℃，载气：氮气，压力：0.3 MPa。结果 十四烷酸、亚麻酸、亚油酸、软脂酸、油酸、十八烷酸分离度均在1.5以上，加样回收率均位于95.3%~103.7%，相对标准偏差(n=6)分别为2.2%，2.0%，1.0%，1.4%，1.4%，2.3%。结论 该方法简便、准确、重复性好，适合油菜蜂花粉原药材中高含量脂肪酸成分的分析测定。 |
| 关键词: 油菜蜂花粉 脂肪酸 高效液相色谱-蒸发光散射检测法 含量测定 |
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| 基金项目:浙江重点企业创新研究院项目；浙江省重大科技专项重点社会发展项目(2012C03005-5)；杭州市产学研合作项目(20122331E35) |
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| Determination of Six Kinds of Fatty Acids in Rape Bee Pollen by HPLC-ELSD |
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XIE Yuanxun1, HE Houhong2, SHENG Weiguo2, YE Jianfeng2, WU Jian2, WANG Ruwei1,2
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1.Pharmaceutical College, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China;2.Zhejiang Institute of TCM＆Natural Drug Co., Ltd., Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine Pharmaceutial Technology, Hangzhou 310052, China
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| Abstract: |
| OBJECTIVE An high-performance liquid chromatographic method with an evaporative light-scattering detector has been developed for the separation and determination of six kinds of fatty acids in rape bee pollen. METHODS An Agilent ZORBAX SB-C18 analytical column(4.6 mm×250 mm, 5 μm) with methanol-acetic acid aqueous solution as mobile phase at a flow rate of 1.2 mL·min-1 were applied. The column temperature was set at 25 ℃. The parameters of ELSD were as follows: the plus was 6.0; the temperature of the drift tube was 30 ℃, the carrier gas pressure was 0.3 MPa. RESULTS The separation degree of stearic, linolenic, linoleic, palm, oleic and myristic acids were above 1.5, and their recoveries ranged from 95.3% to 103.7%, the relative standard deviations(n=6) were 2.2%, 2.0%, 1.0%, 1.4%, 1.4%, 2.3%. CONCLUSION The method is simple, accurate and reproducible, it’s suitable for the simultaneous determination of the six high fatty acids in rape bee pollen. |
| Key words: rape bee pollen fatty acids HPLC-ELSD determination |
