| 引用本文: | 程媛媛,杨传彬,赵佳,荣建辉.硝酸银改性硅胶柱色谱法纯化川芎精油中的藁本内酯[J].中国现代应用药学,2014,31(5):552-555. |
| CHENG Yuanyuan,YANG Chuanbin,ZHAO Jia,RONG Jianhui.Purification of Z-Ligustilide by AgNO3-Impregnated Silica Gel[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(5):552-555. |
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| 摘要: |
| 目的 采用硝酸银改性硅胶分离纯化中药川芎精油的活性化合物藁本内酯。方法 传统硅胶层析柱中加入2%的硝酸银制备一种新型硝酸银改性硅胶层析柱，用石油醚-乙酸乙酯(98.5∶1.5)进行洗脱，采用TLC和UV检测鉴别，将分离制得的藁本内酯用GC-MS进行结构鉴定，并用HPLC峰面积归一法进行纯度检查。结果 从川芎精油中分离得到纯度为98.87%的藁本内酯，可以作为对照品使用。结论 本研究在充分考虑化合物结构特性的基础上，建立了一种流程简单、成本低，藁本内酯纯度高的纯化工艺，可适用于现代化工业生产。 |
| 关键词: 藁本内酯 硝酸银改良硅胶层析柱 分离纯化 气相色谱-质谱法 高效液相色谱法 |
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| Purification of Z-Ligustilide by AgNO3-Impregnated Silica Gel |
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CHENG Yuanyuan, YANG Chuanbin, ZHAO Jia, RONG Jianhui
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School of Chinese Medicine, Li Ka Shing Faculty of Medicine, the University of Hong Kong, Hongkong, China
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| Abstract: |
| OBJECTIVE To investigate the application of AgNO3-impregnated silica gel in a format of column chromatography for the isolation and purification of Z-ligustilide from the essential oil of Ligusticum chuanxiong Hort.. METHODS AgNO3(2%) was impregnated to the common silica gel to generate a new column chromatographic media. The essential oil was eluted with the mobile phase (petroleum ether: acetoacetate=98.5∶1.5), and detected by TLC and UV-Vis spectrometer. The product Z-ligustilide was characterized by GC-MS and HPLC techniques. RESULTS Z-Ligustilide with the purity of 98.87% was effectively purified. CONCLUSION The present study describes a method for rapid isolation of highly pure Z-ligustilide at low cost, allowing future scale-up for modern industrial production. |
| Key words: Z-ligustilide AgNO3-silica gel isolation and purification GC-MS HPLC |
