| 引用本文: | 王涛,刘薇,庄辉传,陈蜜,江振洲.复方甘草酸苷注射液对小鼠放、化疗模型减毒作用实验研究[J].中国现代应用药学,2013,30(9):943-948. |
| WANG Tao,LIU Wei,ZHUANG Huichuan,CHEN Mi,JIANG Zhenzhou.Experimental Research of Toxicity Attenuation of Stronger Neo-minophagen C Injection with Chemotherapy and Radiotherapy in Mice[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(9):943-948. |
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| 摘要: |
| 目的 考察复方甘草酸苷注射液(美能注射液)对于放、化疗动物模型骨髓抑制和免疫损伤的减毒作用，探寻本品临床应用的新价值。方法 选择正常及S180荷瘤小鼠，采用放疗(60Co照射，5.0 Gy)和化疗(环磷酰胺，100 mg·kg-1，腹腔注射)方法建立模型，通过体质量、胸腺指数、脾脏指数、白细胞数和骨髓有核细胞数等指标，评价复方甘草酸苷注射液对放、化疗模型减毒作用。复方甘草酸苷注射液设高、中、低剂量组(以甘草酸苷计分别为40，20，10 mg·kg-1)，1次·d-1，连续静脉注射给药7 d。第8天处死动物，测定体质量、外周血白细胞数、骨髓有核细胞数、胸腺系数、脾脏系数。结果 ①化疗模型中，环磷酰胺可使得正常小鼠和S180荷瘤小鼠胸腺和脾脏的脏器指数显著下降(P<0.01)，白细胞数和骨髓有核细胞数显著减少(P<0.05)。复方甘草酸苷注射液高剂量可明显升高2种化疗动物模型白细胞数和骨髓有核细胞数(P<0.05)，中低剂量组也有部分改善作用。复方甘草酸苷注射液对胸腺和脾脏的脏器指数改善作用不明显。②放疗模型中，60Co照射可使得正常小鼠和S180荷瘤小鼠胸腺和脾脏的脏器指数显著降低(P<0.01)，白细胞和骨髓有核细胞数显著减少(P<0.01)。复方甘草酸苷注射液各剂量组对60Co照射引起的正常小鼠和S180荷瘤小鼠各项指标变化均未见明显改善作用。结论 对于环磷酰胺化疗引起的正常小鼠和S180荷瘤小鼠的骨髓抑制，复方甘草酸苷注射液具有明显的改善作用；对于60Co放疗引起的正常小鼠和S180荷瘤小鼠的骨髓抑制，复方甘草酸苷注射液改善作用不明显，提示本品在化疗骨髓抑制防护方面具有一定的应用价值。 |
| 关键词: 复方甘草酸苷注射液 化疗 放疗 骨髓抑制 |
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| Experimental Research of Toxicity Attenuation of Stronger Neo-minophagen C Injection with Chemotherapy and Radiotherapy in Mice |
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WANG Tao1, LIU Wei2, ZHUANG Huichuan3, CHEN Mi1, JIANG Zhenzhou1
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1.Jiangsu Center for Drug Screening, China Pharmaceutical University, Nanjing 210009, China;2.Department of Pharmacy, Nanjing Occupation Disease Prevention and Tteatment Hospital, Nanjing 210015, China;3.Department of Pharmacy, 81 Hospital of PLA, Nanjing 210002, China
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| Abstract: |
| OBJECTIVE To investigate toxicity attenuation of chemotherapy and radiotherapy with Stronger Neo-Minophagen C Injection(SNMCI) and explore the new value of SNMCI for clinical application. METHODS Normal and S180 inoculated mice were divided randomly into low, medium and high dose groups of SNMCI. SNMCI were intravenously-administrated with the dosage of 40, 20 and 10 mg·kg-1(calculated as dosage of glycyrrhizin) to the groups of mice, respectively, once per day for 7 days. Mice were treated with chemotherapy (cyclophosphamide, 100 mg·kg-1, ip) and radiotherapy(60Co, 5.0 Gy). On 8th day, mice were sacrificed. And then body weight, thymus index, spleen index, periphery blood of WBC, bone marrow nucleated cells were observed. RESULTS ①Chemotherapy could significant reduce thymus index, spleen index, periphery blood of WBC, bone marrow nucleated cells in both normal and S180 inoculated mice(P<0.01). High dose group of SNMCI significantly increased periphery blood of WBC and bone marrow nucleated cells compared with chemotherapy group(P<0.05), medium and low dose groups also had some improvement. Three doses of SNMCI had poor improvement on thymus index and spleen index. ②Radiotherapy could significantly reduce thymus index, spleen index, periphery blood of WBC, bone marrow nucleated cells in both normal and S180 inoculated mice(P<0.01). Three doses of SNMCI had poor improvement on lesion induced by radiotherapy. CONCLUSION SNMCI can significantly improve myelosuppression induced by cyclophosphamide in normal and S180 inoculated mice while has poor improvement for myelosuppression induced by 60Co radiation. The present findings suggest that SNMCI is valuable for myelosuppression induced by chemotherapy. |
| Key words: Stronger Neo-minophagen C injection chemotherapy radiotherapy myelosuppression |
