HPLC测定甲苯磺酸索拉非尼片及其在大鼠血浆中的含量

何春晓; 黄翔; 孙燕雯; 何昱; 张茹萍; 卢红阳; 严叶萍; 张沂平<sup>*</sup>

引用本文:	何春晓,黄翔,孙燕雯,何昱,张茹萍,卢红阳,严叶萍,张沂平.HPLC测定甲苯磺酸索拉非尼片及其在大鼠血浆中的含量[J].中国现代应用药学,2013,30(11):1219-1222.
	HE Chunxiao,HUANG Xiang,SUN Yanwen,HE Yu,ZHANG Ruping,LU Hongyang,YAN Yeping,ZHANG Yiping .Determination of Sorafenib in Tablet and Rat Blood Plasm by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(11):1219-1222.

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HPLC测定甲苯磺酸索拉非尼片及其在大鼠血浆中的含量
何春晓^1,2, 黄翔², 孙燕雯², 何昱², 张茹萍², 卢红阳¹, 严叶萍², 张沂平¹
1.浙江省肿瘤医院化疗中心，杭州 310022;2.浙江中医药大学，杭州 310053

摘要:

目的建立高效液相色谱法测定索拉非尼含量，并探索大鼠血浆中索拉非尼浓度的测定方法。方法采用Eclipse XDB-C₁₈色谱柱(150 mm×4.6 mm，5 μm)；流动相：0.05%甲酸和0.05%三乙胺的水溶液-乙腈(35∶65)；流速：1.0 mL·min^-1；柱温：30 ℃；检测波长：265 nm。结果索拉非尼浓度在0.4~12 μg·mL^-1内线性关系良好，r=0.999 9；加样回收率为100.8%，RSD为1.470%。大鼠按65 mg·kg^-1灌胃给药，给药2.5 h后，其血浆中索拉非尼的药物浓度为2.830 μg·mL^-1。结论本法可用于甲苯磺酸索拉非尼片中药物含量的测定，方法简单、灵敏，同时可检测大鼠血浆中索拉非尼的浓度。

关键词: 高效液相色谱法索拉非尼含量测定

DOI：

分类号:

基金项目:浙江省中医药科学研究基金计划(2012ZA022)；浙江省医药卫生科学研究基金(2007B025)；吴阶平医学基金(320.6750.11059、320.6750.11091、320.6799.1106)

Determination of Sorafenib in Tablet and Rat Blood Plasm by HPLC

HE Chunxiao^1,2, HUANG Xiang², SUN Yanwen², HE Yu², ZHANG Ruping², LU Hongyang¹, YAN Yeping², ZHANG Yiping ¹

1.Department of Medical Oncology, Zhejiang Cancer Hospital, Hangzhou 310022, China;2.Zhejiang Chinese Medical University, Hangzhou 310053, China

Abstract:

OBJECTIVE To establish a high-performance liquid chromatography method for determination of sorafenib, explore a method of measuring the sorafenib concentration in rat blood plasma. METHODS Agilent Eclipse XDB-C₁₈ column (150 mm×4.6 mm, 5 μm) was used. The mobile phase was 35%(0.05% formic acid and 0.05% triethylamine) in aqueous solution and 65% acetonitrile while the flow rate was 1.0 mL·min^-1. The column temperature was 30 ℃. The detection wavelength was 265 nm. RESULTS The standard curve of sorafenib concentration was linear in the range of 0.4-12 μg·mL^-1(r=0.999 9). The average recovery rate of the method was 100.8%, RSD was 1.470%. The rat was treated by gavage with sorafenib at the dosage of 65 mg·kg^-1. The sorafenib concentration in rat blood plasm was 2.830 μg·mL^-1. CONCLUSION The method is suitable for the determination of sorafenib. It is practicable, simple, sensitive, and can be used to measure the sorafenib concentration in rat blood plasma.

Key words: HPLC sorafenib determination