| 引用本文: | 匡荣,陈男,康桦,邓祖跃,王丹,张劲松,朱社敏.山楂叶总黄酮对家兔动脉粥样硬化作用的机理研究[J].中国现代应用药学,2013,30(4):372-375. |
| KUANG Rong,CHEN Nan,KANG Hua,DENG Zuyue,WANG Dan,ZHANG Jinsong,ZHU Shemin.Mechanisms of Total Flavones of Crataegus Leaves on Experimental Atherosclerosis in Rabbits[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(4):372-375. |
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| 摘要: |
| 目的 研究山楂叶总黄酮(total flavones of crataegus leaves，TFCL)对家兔动脉粥样硬化(AS)形成抑制作用的机理。方法 用复方高脂饲料复制家兔AS模型并同时给予TFCL 80 d，取主动脉进行病理切片和大体观察，常规检测血脂和血清中两种抗氧化酶SOD、GSH-PX的活性和NO水平，ELISA法测定C反应蛋白(CRP)、IL-6及TNF-α的量，RT-PCR法检测COX-ⅡmRNA表达。结果 复方高脂饲料饲喂家兔80 d可以形成明显的AS，TFCL在减轻AS的同时能够降低血清中NO、CRP、IL-6及TNF-α的水平，升高SOD和GSH-PX的活性，下调 COX-ⅡmRNA的表达。结论 TFCL对复方高脂饲料形成的家兔AS具有一定的拮抗作用，提高抗氧化酶的活性、抑制炎性因子的生成可能是TFCL抗家兔AS的作用机理。 |
| 关键词: 动脉粥样硬化 山楂叶总黄酮 C反应蛋白 IL-6 TNF-α COX-ⅡmRNA |
| DOI： |
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| 基金项目:浙江省实验动物科技计划项目(2007F80018) |
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| Mechanisms of Total Flavones of Crataegus Leaves on Experimental Atherosclerosis in Rabbits |
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KUANG Rong1,2, CHEN Nan1, KANG Hua2, DENG Zuyue2, WANG Dan2, ZHANG Jinsong2, ZHU Shemin2
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1.College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310014, China;2.Zhejiang Food and Drug Institute for Control Hangzhou, 310004, China
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| Abstract: |
| OBJECTIVE To investigate the mechanisms of total flavones of crataegus leaves (TFCL) on experimental atherosclerosis(AS) in rabbits. METHODS The typical atherosclerosis in rabbits was formed by administrating with high-lipid food or together with TFCL for 80 d. The section of aortia were examined pathologically. The activities of SOD, GSH-PX, the level of NO, CRP, IL-6 and TNF-α in the serum and the relative amount of COX-ⅡmRNA in aortia of atherosclerotic rabbits were detected. RESULTS Atherosclerotic rabbit models were replicated by feeding high-lipid food. TFCL could inhibit the formation of AS with decreasing the level of NO, CRP, IL-6 and TNF-α, increasing the activities of SOD and GSH-PX, down regulating the expression of COX-ⅡmRNA. CONCLUSION The protective effect of TFCL on atherosclerosis may result from elevating the activities of oxygen enzymes but suppressing the production of inflammation factor. |
| Key words: atherosclerosis TFCL CRP IL-6 TNF-α COX-ⅡmRNA |
