| 引用本文: | 闻真,郭桂萍,谢鑫鑫,王俊腾,秦利芳,林东海.细胞膜红色荧光探针PLGA纳米粒的制备及表征[J].中国现代应用药学,21013,(3):280-284. |
| WEN Zhen,GUO Guiping,XIE Xinxin,WANG Junteng,QIN Lifang,LIN Donghai .Preparation and Characterization of DiI Loaded PLGA Nanoparticles[J].Chin J Mod Appl Pharm(中国现代应用药学),21013,(3):280-284. |
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| 摘要: |
| 目的 以聚乳酸-羟基乙酸共聚物(PLGA)和单甲氧基聚乙二醇聚乳酸-羟基乙酸共聚物(MePEG-PLGA)为材料，制备包载细胞膜红色荧光探针(DiI)的纳米粒，为后续的细胞实验奠定基础。方法 采用自乳化溶剂扩散法制备纳米粒；超滤法分离纯化纳米粒，紫外可见分光光度法测定DiI的含量，并计算包封率。结果 DiI用量为0.5 mg，PLGA或MePEG- PLGA投入量为50 mg，PVA浓度为0.5%，可制得粒径较合适的纳米粒。DiI-PLGA-NP平均粒径为(280.7±3.6)nm，Zeta电位为(-2.98±0.47)mV，包封率可达88.0%； DiI-MePEG-PLGA-NP平均粒径为(157.2±3.2)nm，Zeta电位为(-4.90±0.54)mV，包封率可达87.1%。结论 DiI作为脂溶性良好的示踪剂，用其制得的荧光素纳米粒可为后续的体外实验奠定良好的基础。 |
| 关键词: 细胞膜红色荧光探针 聚乳酸-羟基乙酸共聚物纳米粒 单甲氧基聚乙二醇聚乳酸-羟基乙酸共聚物纳米粒 自乳化溶剂扩散法 超滤法 紫外可见分光光度法 |
| DOI： |
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| 基金项目:国家自然科学基金资助项目(30973949)；山东省自然科学基金资助项目(ZR2009CM012) |
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| Preparation and Characterization of DiI Loaded PLGA Nanoparticles |
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WEN Zhen, GUO Guiping, XIE Xinxin, WANG Junteng, QIN Lifang, LIN Donghai
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Department of Pharmacy, Yantai University, Yantai 264005, China
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| Abstract: |
| OBJECTIVE To prepare DiI loaded nanoparticles using PLGA and MePEG-PLGA as carriers. METHODS Spontaneous emulsion solvent diffusion method(SESD) was adopt to prepare nanoparticles. Ultrafiltration-UV-visible spectrophotometry was used for the determination of DiI-NP' encapsulation efficiency. RESULTS When DiI’s dosage was 0.5 mg, PLGA’s or MePEG-PLGA’s dosage was 50 mg, PVA’s content was 0.5%, the suitable nanoparticles were obtained. DiI-PLGA-NP’ average diameter was (280.7±3.6)nm, DiI-PLGA-NP’ Zeta potential was (-2.98±0.47)mV, DiI-PLGA-NP’ encapsulation efficiency was 88.0%; DiI-MePEG-PLGA-NP’ average diameter was (157.2±3.2)nm, DiI-MePEG-PLGA-NP’ Zeta potential was (-4.90±0.54)mV, DiI-MePEG-PLGA-NP’ encapsulation efficiency was 87.1%. CONCLUSION As a liposoluble long-term tracer, DiI-NP can lay the foundation for subsequent in vitro tests. |
| Key words: fluorescent probe(DiI) PLGA nanoparticles MePEG-PLGA nanoparticles spontaneous emulsion solvent diffusion method ultrafiltration UV-visible spectrophotometry |
