| 引用本文: | 惠毅,魏海梁,闫曙光,李京涛,史捷.血管活性肠肽对肺泡巨噬细胞M1/M2型极化及相关细胞因子的影响[J].中国现代应用药学,2021,38(17):2053-2059. |
| HUI Yi,WEI Hailiang,YAN Shuguang,LI Jingtao,SHI Jie.Effects of Vasoactive Intestinal Peptide on M1/M2 Polarization and Related Cytokines in Alveolar Macrophages[J].Chin J Mod Appl Pharm(中国现代应用药学),2021,38(17):2053-2059. |
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| 摘要: |
| 目的 观察血管活性肠肽（vasoactive intestinal peptide，VIP）对肺泡巨噬细胞（alveolar macrophages，AM） M1/M2极化和相关细胞因子的影响。方法 分别采用脂多糖（lipopolysaccharide，LPS）联合γ-干扰素（interferon-γ，IFN-γ）、白细胞介素-4（interleukin 4，IL-4）联合白细胞介素-13（interleukin 13，IL-13）诱导AM M1/M2型极化，后用10-8~10-6 mol·L-1 VIP干预极化的AM，免疫荧光双标法测定M1型AM标记物CD86和促炎因子白细胞介素-6（interleukin 6，IL-6）的共表达，M2型AM标记物CD206和抗炎因子白细胞介素-10（interleukin 10，IL-10）的共表达；ELISA检测细胞上清中IL-6、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）、IL-10、精氨酸酶-1（arginase-1，Arg-1）的表达，RT-PCR检测巨噬细胞表面标记物CD86、CD206和巨噬细胞相关因子IL-6、TNF-α、诱导型一氧化氮合酶（inducible nitric oxide synthase，iNOS）、IL-10、Arg-1、几丁质酶-3样蛋白3（chitinase 3-like 3，Ym1）的表达。结果 经LPS联合IFN-γ、IL-4联合IL-13诱导后，AM分别向M1/M2型极化，与M1型相关的促炎因子IL-6、TNF-α、iNOS和与M2型相关的抗炎因子IL-10、Arg-1、Ym1的激活和释放明显高于未经诱导的正常组（P<0.05）；不同浓度VIP （10-8~10-6 mol·L-1）的干预，均可下调M1型AM和相关炎症因子IL-6、TNF-α、iNOS的活性和表达（P<0.05）；上调M2型AM和相关抗炎因子IL-10、Arg-1、Ym1的活性和表达（P<0.05）。结论 VIP可抑制AM M1型极化，减少炎症因子IL-6、TNF-α、iNOS的激活和释放；促进AM M2型极化，提高抗炎因子IL-10、Arg-1、Ym1的激活和释放，提示VIP在肺内炎症时可能通过调控AM M1/M2型极化，抑制炎症反应，对肺组织起保护性作用。 |
| 关键词: 血管活性肠肽 肺泡巨噬细胞 M1/M2型巨噬细胞极化 炎症因子 |
| DOI：10.13748/j.cnki.issn1007-7693.2021.17.002 |
| 分类号:R966 |
| 基金项目:国家自然科学基金项目（81703974）；陕西中医药大学学科创新团队建设项目（2019-YL05） |
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| Effects of Vasoactive Intestinal Peptide on M1/M2 Polarization and Related Cytokines in Alveolar Macrophages |
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HUI Yi1, WEI Hailiang2, YAN Shuguang1, LI Jingtao3, SHI Jie4
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1.College of Basic Medicine, Shaanxi University of Chinese Medicine, Xianyang 712046, China;2.Department of General Surgery, The Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712000, China;3.Department of Infectious Disease, The Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712000, China;4.Department of Respiratory, The Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712000, China
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| Abstract: |
| OBJECTIVE To observe the effects of vasoactive intestinal peptide(VIP) on M1/M2 polarization and related cytokines of alveolar macrophages(AM). METHODS Lipopolysaccharide(LPS) combined with interferon-γ(IFN-γ) and interleukin-4(IL-4) combined with interleukin-13(IL-13) were used to induce M1/M2 type polarization of AM, and then VIP (10-8-10-6 mol·L-1) was used to intervene the polarization of AM. The co-expression of M1 type AM marker CD86 and pro-inflammatory factor interleukin 6(IL-6) and co-expression of M2-type AM marker CD206 and anti-inflammatory factor interleukin 10(IL-10) was determined by immunofluorescence double standard method. Expression of IL-6, tumor necrosis factor-α(TNF-α), IL-10 and arginase-1(Arg-1) in cell supernatant was detected by ELISA. The macrophage surface markers CD86, CD206 and macrophage related factors IL-6, TNF-α, inducible nitric oxide synthase(iNOS), IL-10, Arg-1 and chitinase 3-like 3(Ym1) were detected by RT-PCR. RESULTS After induction by LPS combined with IFN-γ and IL-4 combined with IL-13, AM was polarized to M1/M2 type respectively. The activation and release of pro-inflammatory factors IL-6, TNF-α and iNOS related to M1 and anti-inflammatory factors IL-10, Arg-1 and Ym1 related to M2 were significantly higher than those in the uninduced normal group(P<0.05). Interference with different concentrations of VIP(10-8-10-6 mol·L-1) could down-regulate the activity and expression of M1 type AM and related inflammatory cytokines IL-6, TNF-α and iNOS(P<0.05). The activity and expression of M2 type AM and related anti-inflammatory factors IL-10, Arg-1 and Ym1 were up-regulated(P<0.05). CONCLUSION VIP can inhibit AM M1-type polarization and reduce the activation and release of inflammatory cytokines IL-6, TNF-α and iNOS. It can promote AM M2-type polarization and increase the activation and release of anti-inflammatory factors IL-10, Arg-1 and Ym1, suggesting that VIP may inhibit the inflammatory response and play a protective role in lung tissue by regulating the M1/M2-type polarization of AM in the process of pulmonary inflammation. |
| Key words: vasoactive intestinal peptide alveolar macrophages polarization of M1/M2 macrophages inflammatory cytokines |
