• 首页期刊简介编委会刊物订阅专栏专刊电子刊广告合作联系我们English
引用本文:杜正彩,俞航萍,陈章美,夏中尚,赵芳惠,梁云飞,徐懿乔,郝二伟,侯小涛,邓家刚.中华跌打丸对斑马鱼骨折愈合作用研究[J].中国现代应用药学,2021,38(12):1454-1460.
DU Zhengcai,YU Hangping,CHEN Zhangmei,XIA Zhongshang,ZHAO Fanghui,LIANG Yunfei,XU Yiqiao,HAO Erwei,HOU Xiaotao,DENG Jiagang.Study on Effect of Zhonghua Dieda Pill on Fracture Healing of Zebrafish[J].Chin J Mod Appl Pharm(中国现代应用药学),2021,38(12):1454-1460.
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 1131次   下载 666 本文二维码信息
码上扫一扫!
分享到: 微信 更多
中华跌打丸对斑马鱼骨折愈合作用研究
杜正彩1, 俞航萍2, 陈章美1, 夏中尚3, 赵芳惠1, 梁云飞4, 徐懿乔2, 郝二伟5, 侯小涛5, 邓家刚5
1.广西中医药大学, 科学实验中心, 南宁 530200;2.杭州环特生物科技股份有限公司, 杭州 310051;3.成都中医药大学, 成都 611137;4.广西梧州制药(集团)股份有限公司, 广西 梧州 543000;5.广西中医药大学, 广西中药药效研究重点实验室, 南宁 530200
摘要:
目的 应用斑马鱼研究中华跌打丸促骨折愈合、抗炎、促血管形成及软骨损伤修复作用。方法 采用顿性棱刀挤压斑马鱼尾鳍建立骨折模型,评价其骨折愈合及抗炎作用;用转基因血管荧光斑马鱼评价其促血管形成作用;地塞米松建立斑马鱼软骨损伤模型,评价其软骨损伤修复作用;通过Q-PCR检测中华跌打丸对TNF-αIL-1βBMP-2BMP-7基因表达的影响。结果 与模型对照组比较,中华跌打丸在浓度为每尾2 mg时,骨折愈合促进作用为20.8%(P<0.05);浓度在15.6,31.3和62.5 mg·mL-1时炎症消退作用分别为44.6%,50.6%和52.6%(P<0.05),且伴随TNF-αIL-1β基因的表达有不同程度下调(P<0.05或P<0.01);浓度为1 000,2 000 mg·L-1时,促血管形成作用分别为19.7%和49.9%(P<0.01或P<0.001);中华跌打丸通过上调BMP-2BMP-7基因的表达起到软骨修复作用(P<0.01或P<0.001),浓度为500 mg·L-1时,软骨损伤修复作用为42.0%(P<0.05)。结论 中华跌打丸对斑马鱼有促进骨折愈合、抗炎、促血管及软骨损伤修复作用,且通过下调TNF-αIL-1β的表达起到消炎作用,上调BMP-2BMP-7基因的表达起到软骨修复作用。
关键词:  中华跌打丸|斑马鱼|骨折愈合|抗炎|促血管形成|软骨损伤修复
DOI:10.13748/j.cnki.issn1007-7693.2021.12.008
分类号:R285.5
基金项目:广西科技重大专项(桂科AA17202040-2);广西中医药大学校级重点硕士研究生科研创新项目(YCSZ20190022);广西中药药效研究重点实验室项目(19-050-39);广西农作物废弃物功能成分研究协同创新中心建设项目
Study on Effect of Zhonghua Dieda Pill on Fracture Healing of Zebrafish
DU Zhengcai1, YU Hangping2, CHEN Zhangmei1, XIA Zhongshang3, ZHAO Fanghui1, LIANG Yunfei4, XU Yiqiao2, HAO Erwei5, HOU Xiaotao5, DENG Jiagang5
1.Guangxi University of Traditional Chinese Medicine, Guangxi Scientific Experimental Center of Traditional Chinese Medicine, Nanning, 530200, China;2.Hunter Biotechnology, Co., Ltd, Hangzhou 310051, China;3.Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China;4.Guangxi Wuzhou Pharmaceutical (Group) Co., Ltd, Wuzhou, 543000, China;5.Guangxi University of Traditional Chinese Medicine, Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica, Nanning, 530200, China
Abstract:
OBJECTIVE To study the antifracture healing, anti-inflammatory, angiogenesis and repair of cartilage damage effects of Zhonghua Dieda pill by using zebrafish. METHODS The fracture model was established by squeezing the tail fin of zebrafish by a blunt edge knife, and the effect of Zhonghua Dieda pill on fracture healing and anti-inflammatory was then evaluated; transgenic vascular fluorescence zebrafish was used to evaluate the angiogenic effect; cartilage damage model was established by treating zebrafish with dexamethasone, and then the effect of cartilage damage reparing was evaluated; the effects of Zhonghua Dieda pill on the gene expressions of TNF-α, IL-1β, BMP-2 and BMP-7 were measured by Q-PCR. RESULTS Compared with the model control group, the fracture healing effect of the Zhonghua Dieda pill was 20.8%(P<0.05) when concentration was 2 mg per tail. With down-regulating the expression of TNF-α and IL-1β genes(P<0.05 or P<0.01), the anti-inflammatory effect of Zhonghua Dieda pill was 44.6%, 50.6% and 52.6% at the 15.6, 31.3 and 62.5 mg·mL-1 concentration(P<0.05); the angiogenesis effect of Zhonghua Dieda pill was 19.7% and 49.9% at the 1 000, 2 000 mg·L-1 concentration groups(P<0.01 or P<0.001); Zhonghua Dieda pill showed cartilage damage repairing effect by up-regulating the expression of BMP-2 and BMP-7 genes and result in 42.0% effect of cartilage damage repairing on zebrafish model at 500 mg·mL-1 concentration(P<0.05). CONCLUSION Zhonghua Dieda pill can promote fracture healing, anti-inflammatory, angiogenesis and cartilage damage repairing in zebrafish, and play an anti-inflammatory role by down-regulating the expression of TNF-α and IL-1β and play a cartilage repairing by up-regulating the expression of BMP-2 and BMP-7 genes.
Key words:  Zhonghua Dieda pill|zebrafish|fracture healing|anti-inflammatory|angiogenesis
扫一扫关注本刊微信