| 引用本文: | 汪霞,林丽琴,杨直,高丽琼,王秀秀,金朦娜,陈鸿健.HPLC-MS/MS测定化妆品中14种荧光增白剂[J].中国现代应用药学,2023,40(23):3236-3244. |
| WANG Xia,LIN Liqin,YANG Zhi,GAO Liqiong,WANG Xiuxiu,JIN Mengna,CHEN Hongjian.Determination of Fourteen Fluorescent Whitening Agents in Cosmetics by HPLC-MS/MS[J].Chin J Mod Appl Pharm(中国现代应用药学),2023,40(23):3236-3244. |
|
| 摘要: |
| 目的 建立HPLC-MS/MS定量测定化妆品中14种荧光增白剂的分析方法。方法 样品经N,N-二甲基甲酰胺超声提取，选用Waters ACQUITY UPLC ®BEH C18(2.1 mm×50 mm，1.7 μm)色谱柱，以甲醇-0.1%氨水水溶液梯度洗脱，流速0.3 mL·min-1，柱温40 ℃；质谱法采用三重四级杆质谱，电喷雾离子源，在正/负离子、多反应监测模式下进行扫描。结果 14种荧光增白剂在定量范围内线性关系良好，相关系数均＞0.99，定量限为0.01~20 μg·g-1，检出限为0.004~ 8 μg·g-1；在3个添加水平下的平均回收率为85.4%~108.9%，相对标准偏差为0.3%~7.2%。结论 该方法灵敏度高，专属性强，操作简单方便，适用于化妆品中14种荧光增白剂的含量测定。 |
| 关键词: 超高效液相色谱-串联质谱法 荧光增白剂 化妆品 |
| DOI：10.13748/j.cnki.issn1007-7693.20230266 |
| 分类号:R917 |
| 基金项目:浙江省药品监管系统科技计划项目(2021026) |
|
| Determination of Fourteen Fluorescent Whitening Agents in Cosmetics by HPLC-MS/MS |
|
WANG Xia, LIN Liqin, YANG Zhi, GAO Liqiong, WANG Xiuxiu, JIN Mengna, CHEN Hongjian
|
|
Hangzhou Institute for Food and Drug Control, Hangzhou 310022, China
|
| Abstract: |
| OBJECTIVE To establish a method for the determination of fourteen fluorescent whitening agents in cosmetics by HPLC-MS/MS. METHODS Samples were extracted on a Waters ACQUITY UPLC ®BEH C18(2.1 mm×50 mm, 1.7 μm) column after ultrasonic extracted by DMF with the mobile phase consisted of methanol-0.1% ammonia water solution by gradient elution. The flow rate was 0.3 mL·min-1. The column temperature was 40 ℃. MS was performed using triple quadrupole mass spectrometry. Electrospray ionization source was operated in the positive/negative mode using multiple reaction monitoring scanning mode. RESULTS The results showed that there were good linear relationships for the fluorescent whitening agents in a certain concentration range with correlation coefficients(r) greater than 0.99. The limits of quantification were 0.01-20 μg·g-1 and the limits of detection were 0.004-8 μg·g-1. The average recoveries at three spiked levels were in the range of 85.4%-108.9%, and the relative standard deviation were in the range of 0.3%-7.2%. CONCLUSION The method has high sensitivity, strong specificity, simple and convenient operation, and is suitable for the detection of fourteen fluorescent whitening agents in cosmetics. |
| Key words: HPLC-MS/MS fluorescent whitening agents cosmetic |
