| 引用本文: | 褚悟男,李翔,韩玲玲,蒲婧哲,翟宏焱,王浩,张亚中.UHPLC-MS/MS同时测定白芷中34种植物生长调节剂残留量[J].中国现代应用药学,2023,40(6):783-791. |
| CHU Wunan,LI Xiang,HAN Lingling,PU Jingzhe,ZHAI Hongyan,WANG Hao,ZHANG Yazhong.Simultaneous Determination of 34 Plant Growth Regulator Residues in Angelicae Dahuricae Radix by UHPLC-MS/MS[J].Chin J Mod Appl Pharm(中国现代应用药学),2023,40(6):783-791. |
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| UHPLC-MS/MS同时测定白芷中34种植物生长调节剂残留量 |
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褚悟男1, 李翔2,3, 韩玲玲2,3, 蒲婧哲2,3, 翟宏焱2,3, 王浩2,3, 张亚中2,3,4
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1.安徽中医药大学药学院, 合肥 230012;2.安徽省食品药品检验研究院, 合肥 230051;3.国家药监局中药质量研究与评价重点实验室, 合肥 230051;4.中药饮片制造新技术安徽省重点实验室, 合肥 230051
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| 摘要: |
| 目的 基于UHPLC-MS/MS建立同时测定白芷中34种植物生长调节剂残留量的分析方法。方法 样品通过乙腈高速匀浆提取，浓缩后直接进样，采用Waters Acquity UPLC HSS T3(2.1 mm×150 mm，1.8 µm)色谱柱，流动相0.1%甲酸水溶液含10 mmol·L–1甲酸铵(A)-乙腈(B)，用基质匹配标液外标法进行定量。结果 34种植物生长调节剂在基质中的线性关系良好，相关系数均>0.995，检出限为0.01~9.26 µg·kg-1，平均回收率为72.4%~100.6%，RSD均<15%。采用该法对35批白芷样品中的植物生长调节剂残留情况进行筛查，其中19批样品中检测出6种植物生长调节剂。结论 建立的植物生长调节剂多残留检测方法灵敏度高，准确性好，有针对性，且前处理简单快速，可用于筛查和检测白芷中植物生长调节剂多残留量。 |
| 关键词: 植物生长调节剂 白芷 残留量 超高效液相色谱-串联质谱法 |
| DOI：10.13748/j.cnki.issn1007-7693.20222581 |
| 分类号:R284.1 |
| 基金项目:国家药典委员会药品标准制修订课题(2021Z04) |
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| Simultaneous Determination of 34 Plant Growth Regulator Residues in Angelicae Dahuricae Radix by UHPLC-MS/MS |
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CHU Wunan1, LI Xiang2,3, HAN Lingling2,3, PU Jingzhe2,3, ZHAI Hongyan2,3, WANG Hao2,3, ZHANG Yazhong2,3,4
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1.School of Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei 230012, China;2.Anhui Institute of Food and Drug Control, Hefei 230051, China;3.NMPA Key Laboratory for Quality Research and Evaluation of Traditional Chinese Medicine, Hefei 230051, China;4.Anhui Provincial Key Laboratory of New Manufacturing Technology for Traditional Chinese Medicine Decoction Pieces, Hefei 230051, China
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| Abstract: |
| OBJECTIVE To establish an analytical method for the simultaneous determination of 34 plant growth regulators residues in Angelicae Dahuricae Radix based on UHPLC-MS/MS. METHODS The samples were extracted by acetonitrile high-speed homogenization, concentrated, injected directly without purification method, separated by a Waters Acquity UPLC HSS T3(2.1 mm×150 mm, 1.8 µm) column with mobile phase of 0.1% formic acid aqueous solution containing 10 mmol·L-1 ammonium formate(A)-acetonitrile(B). The matrix-matched external standard method was used for quantitative analysis. RESULTS The calibration curves of 34 plant growth regulators showed good linearity with correlation coefficients >0.995. The limits of detection were 0.01-9.26 µg·kg-1, and average recoveries of 72.4%-100.6%, the RSD <15%. The plant growth regulators in 35 batches of Angelicae Dahuricae Radix were analyzed by this method. CONCLUSION The established method is sensitive, accurate, targeted, and simple with rapid pre-treatment, and can be used for screening and detection of plant growth regulator multi-residues in Angelicae Dahuricae Radix. |
| Key words: plant growth regulator Angelicae Dahuricae Radix residue UHPLC-MS/MS |
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