UHPLC-MS/MS同时测定鳖甲煎丸中鳖甲胶和阿胶的特征肽含量

王倩; 刘宇文<sup>*</sup>; 张杭倩

引用本文:	王倩,刘宇文,张杭倩.UHPLC-MS/MS同时测定鳖甲煎丸中鳖甲胶和阿胶的特征肽含量[J].中国现代应用药学,2022,39(16):2120-2124.
	WANG Qian,LIU Yuwen,ZHANG Hangqian.Simultaneous Determination of Marker Peptides of Trionycis-shell Glue and Asini Corii Colla in Biejiajian Pills by UHPLC-MS/MS[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(16):2120-2124.

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UHPLC-MS/MS同时测定鳖甲煎丸中鳖甲胶和阿胶的特征肽含量
王倩¹, 刘宇文², 张杭倩¹
1.杭州方回春堂科技服务有限公司, 杭州 310000;2.杭州市食品药品检验研究院, 杭州 310000

摘要:

目的建立同时测定鳖甲煎丸中鳖甲胶和阿胶特征肽含量的分析方法,为鳖甲煎丸的质量控制提供技术支持。方法采用胰蛋白酶对鳖甲煎丸提取溶液进行酶解,利用超高效液相色谱-串联质谱法(UHPLC-MS/MS),在电喷雾电离(ESI)正离子模式下采用多反应监测(multiple reaction monitoring,MRM),用外标法对鳖甲胶和阿胶的特征多肽进行含量测定。结果方法学研究表明,该方法具有较好的专属性、精密度、稳定性、重复性。鳖甲特征肽A、驴源多肽A₁、驴源多肽A₂在线性范围内线性关系良好,相关系数均>0.999。3种特征多肽的平均回收率为97.08%~105.10%。结论所建立的方法可用于鳖甲煎丸中鳖甲胶和阿胶的质量控制。鳖甲煎丸中鳖甲胶、阿胶的特征多肽含量低于拟定限度,应引起有关单位的重视。

关键词: 鳖甲胶阿胶超高效液相色谱-串联质谱法多肽含量

DOI：10.13748/j.cnki.issn1007-7693.2022.16.011

分类号:R281.4

基金项目:浙江省药品监督管理局科技计划项目(2022003)

Simultaneous Determination of Marker Peptides of Trionycis-shell Glue and Asini Corii Colla in Biejiajian Pills by UHPLC-MS/MS

WANG Qian¹, LIU Yuwen², ZHANG Hangqian¹

1.Hangzhou Fang Hui Chun Tang Technology Service Co., Ltd., Hangzhou 310000, China;2.Hangzhou Institute for Food and Drug Control, Hangzhou 310000, China

Abstract:

OBJECTIVE To establish an analytical method for simultaneous determination of marker peptides contents of trionycis-shell glue and Asini Corii Colla in Bejiajian pills,and provide technical support for quality control of Bejiajian pills.METHODS Biejiajian pills was enzymolized by trypsin,the detection was performed by UHPLC-MS/MS in the ESI positive mode and MRM mode.The content of marker peptides in trionycis-shell glue and Asini Corii Colla was determined by external standard method.RESULTS The methodological evaluation showed that the method had good specificity,precision,stability,repeatability.Good linear relationships of trionychid marker peptide A,donkey polypeptide A₁ and donkey polypeptide A₂ were obtained and the correlation coefficients (r) were>0.999.The average recoveries of the 3 marker peptides were in the range of 97.08%-105.10%.CONCLUSION The established method can be used for the quality control of trionycis-shell gelatin and Asini Corii Colla in Biejiajian pills.Relevant units should pay attention to the content of marker polypeptide of trionycis-shell glue and Asini Corii Colla in Biejiajian pills is lower than the proposed limit.

Key words: trionycis-shell glue Asini Corii Colla UHPLC-MS/MS polypeptide content