| 引用本文: | 邓志鹏,柳佳,熊山,田海涛.UPLC-Q-Exactive Plus Orbitrap-MS鉴定桑色素在大鼠体内的代谢产物[J].中国现代应用药学,2022,39(24):3262-3266. |
| DENG Zhipeng,LIU Jia,XIONG Shan,TIAN Haitao.Metabolite Identification of Morin in Rats by UPLC-Q-Exactive Plus Orbitrap-MS[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(24):3262-3266. |
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| 摘要: |
| 目的 利用UPLC-Q-Exactive Plus Orbitrap-MS技术对桑色素在大鼠体内的主要代谢产物进行研究。方法 大鼠灌胃给予桑色素20 mg·kg-1后,分别收集血浆、尿液和粪便样品,采用UPLC-Q-Exactive Plus Orbitrap-MS技术测定桑色素的体内代谢物。结果 根据一级质谱分子离子信息和二级质谱碎裂离子信息,在大鼠血浆和尿液中均发现2个葡萄糖醛酸代谢产物,在粪便中发现脱氢产物。结论 桑色素在大鼠体内的主要代谢途径为葡萄糖醛酸化反应。本研究初步阐明了桑色素在大鼠体内的代谢情况,为进一步药理作用机制研究提供依据。 |
| 关键词: 桑色素 代谢产物 UPLC-Q-Exactive Plus Orbitrap-MS |
| DOI：10.13748/j.cnki.issn1007-7693.2022.24.011 |
| 分类号:R284.1 |
| 基金项目:山东省自然科学基金项目(ZR2019MH051) |
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| Metabolite Identification of Morin in Rats by UPLC-Q-Exactive Plus Orbitrap-MS |
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DENG Zhipeng1, LIU Jia2, XIONG Shan2, TIAN Haitao1
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1.School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China;2.Institute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250062, China
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| Abstract: |
| OBJECTIVE To study the metabolites of morin in rats in vivo by UPLC-Q-Exactive Plus Orbitrap-MS. METHODS Rat plasma, urine and feces samples were collected after oral administration of 20 mg·kg-1 morin, and the metabolites of morin in vivo were detected by UPLC-Q-Exactive Plus Orbitrap-MS. RESULTS Two glucuronidation metabolites were observed in rat plasma and urines, and one dehydrogenation metabolite was in feces according to the MS and MS/MS information. CONCLUSION The main metabolic pathway of morin is glucuronidation in rats. This preliminary study clarified the metabolism of morin in rats, and provided the basis for further study of pharmacological mechanism. |
| Key words: morin metabolite UPLC-Q-Exactive Plus Orbitrap-MS |
