| 引用本文: | 杨海军,刘睿,陈盛君,李松,韩疏影.基于超声辅助酶解与串联质谱联用的鹿角特征肽检测[J].中国现代应用药学,2022,39(19):2543-2548. |
| YANG Haijun,LIU Rui,CHEN Shengjun,LI Song,HAN Shuying.Detection of Deer Horn Specific Peptides Using Ultrasonic Assisted Digestion Combined with Tandem Mass Spectrometry[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(19):2543-2548. |
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| 基于超声辅助酶解与串联质谱联用的鹿角特征肽检测 |
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杨海军1, 刘睿2,3, 陈盛君4, 李松4, 韩疏影2,3
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1.江苏卫生健康职业学院, 南京 211800;2.南京中医药大学, 江苏省中药资源产业化过程协同创新中心/中药资源产业化与方剂创新药物国家地方联合工程研究中心, 南京 210023;3.南京中医药大学, 药学院, 南京 210023;4.江阴天江药业有限公司, 江苏 江阴 214434
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| 摘要: |
| 目的 检测鹿角特征肽类成分，为鹿角、鹿角胶及其相关产品的质量研究提供实验依据。方法 通过超声辅助酶解的方法，快速酶解鹿角、鹿角胶样品。采用高效液相色谱-三重四极杆质谱对酶解液进行检测，在电喷雾离子化的正离子模式下，采用多反应监测，以鹿角特征肽m/z 765.5→554.4、m/z 850.4→515.4、m/z 864.0→535.4、m/z 858.7→527.4、m/z 612.2→464.2、m/z 845.0→507.3为离子对，检测鹿角、鹿角胶及掺伪样品的鹿角特征肽。结果 该方法可在1 h内完成鹿角相关样品特征肽类成分的检测，可用于鹿角与鹿皮样品的区分以及掺伪鹿角样品的检测。结论 采用超声辅助酶解与质谱联用的方法快速、准确、专属性强，可用于鹿角相关样品的快速精准检测。 |
| 关键词: 鹿角 鹿角胶 配方颗粒 超声辅助酶解 串联质谱 特征肽 |
| DOI：10.13748/j.cnki.issn1007-7693.2022.19.017 |
| 分类号:R284.1 |
| 基金项目:南京中医药大学中药学一流学科开放课题(2020YLXK009) |
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| Detection of Deer Horn Specific Peptides Using Ultrasonic Assisted Digestion Combined with Tandem Mass Spectrometry |
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YANG Haijun1, LIU Rui2,3, CHEN Shengjun4, LI Song4, HAN Shuying2,3
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1.Jiangsu Health Vocational College, Nanjing 211800, China;2.Nanjing University of Chinese Medicine, Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization/Formulae Innovative Medicine, Nanjing 210023, China;3.Nanjing University of Chinese Medicine, School of Pharmacy, Nanjing 210023, China;4.Jiangyin Tianjiang Pharmaceutical Co., Ltd., Jiangyin 214434, China
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| Abstract: |
| OBJECTIVE To detect Cervi Cornu specific peptides components,provide an experimental basis for the quality standard investigation on Cervi Cornu,Cervi Cornu Colla and their products. METHODS By using ultrasonic assisted trypsin digestion method,Cervi Cornu,and Cervi Cornu Colla samples were prepared rapidly.High performance liquid chromatography with triple-quadrupole mass spectrometry was used to detect the Cervi Cornu and Cervi Cornu Colla samples.Under positive electrospray ionization mode and multiple reaction monitoring,ion pair of m/z 765.5→554.4,m/z 850.4→515.4,m/z 864.0→535.4,m/z 858.7→527.4,m/z 612.2→464.2 and m/z 845.0→507.3 were used to detect deer specific peptides in Cervi Cornu,Cervi Cornu Colla or adulterated Cervi Cornu Colla samples.RESULTS The determination of deer specific peptides in Cervi Cornu samples could be achieved in 1 h used the present method,which could be used to distinguish Cervi Cornu and deer skin samples,and the detection of adulterated Cervi Cornu Colla samples.CONCLUSION Ultrasonic assisted digestion combined with tandem mass spectrometry is fast,accurate and specific.It can be used for the rapid and accurate detection of Cervi Cornu,Cervi Cornu Colla and their products. |
| Key words: Cervi Cornu Cervi Cornu Colla dispensing granule ultrasonic assisted digestion tandem mass spectrometry specific peptides |
