| 引用本文: | 樊慧杰,李艳荣,柴智,刘建春,殷福栋,王新亮,闫玉清,肖保国,马存根.FSD-C10抑制MPTP诱导的帕金森病小鼠氧化应激及炎性反应研究[J].中国现代应用药学,2022,39(3):294-299. |
| FAN Huijie,LI Yanrong,CHAI Zhi,LIU Jianchun,YIN Fudong,WANG Xinliang,YAN Yuqing,XIAO Baoguo,MA Cungen.Study on FSD-C10 Inhibiting Oxidative and Inflammatory Responses in MPTP Induced Parkinson's Disease Mice[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(3):294-299. |
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| FSD-C10抑制MPTP诱导的帕金森病小鼠氧化应激及炎性反应研究 |
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樊慧杰1, 李艳荣1, 柴智1, 刘建春1, 殷福栋1, 王新亮1, 闫玉清2, 肖保国3, 马存根1,2
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1.山西中医药大学神经生物学研究中心/国家中医药管理局多发性硬化益气活血重点研究室, 山西 晋中 030619;2.山西大同大学脑科学研究所, 山西 大同 037009;3.复旦大学华山医院神经病学研究所, 上海 200025
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| 摘要: |
| 目的 研究口服FSD-C10对帕金森病（Parkinson's disease，PD）小鼠的治疗作用，并探讨其相关机制。方法 小鼠随机分为正常组、PD组和FSD-C10组（50 mg·kg-1），旷场行为学测试评估小鼠移动的总距离、休息时间、穿格次数；Western blotting检测脑组织酪氨酸羟化酶（tyrosine hydroxylase，TH）、ROCKⅡ；比色法检测脑组织过氧化氢酶（catalase，CAT）、还原型谷胱甘肽（glutathione，GSH）、丙二醛和一氧化氮（nitric oxide，NO）；ELISA检测脑组织肿瘤坏死因子（tumor necrosis factor-α，TNF-α）、白介素-1β（interleukin-1β，IL-1β）及白介素-6（interleukin-6，IL-6）。结果 与正常组比较，PD组小鼠移动的总距离、穿格次数降低（P<0.01），休息时间延长（P<0.01），TH表达降低（P<0.05），ROCKⅡ表达增高（P<0.05），CAT活力下降（P<0.05），GSH含量降低（P<0.01），丙二醛含量升高（P<0.05），TNF-α、IL-1β、IL-6及NO升高（P<0.05或P<0.01）。与PD组比较，FSD-C10组小鼠移动的总距离、穿格次数增加（P<0.05），休息时间缩短（P<0.01），TH表达增高（P<0.05），ROCKⅡ表达降低（P<0.05），CAT活力增加（P<0.01），GSH含量升高（P<0.01），丙二醛含量降低（P<0.01），TNF-α、IL-1β、IL-6及NO下降（P<0.01）。结论 口服FSD-C10可以改善PD小鼠的行为学表现，对PD有明确的治疗作用，其作用机制可能与恢复氧化-抗氧化体系平衡、减少炎性因子分泌相关。 |
| 关键词: 帕金森病 FSD-C10 氧化应激 抗炎作用 |
| DOI：10.13748/j.cnki.issn1007-7693.2022.03.002 |
| 分类号:R965.1 |
| 基金项目:国家自然科学基金项目（81703978）；中央引导地方科技发展资金自由探索类基础研究项目（YDZX20201400001483）；山西省青年拔尖人才支持计划项目（晋组办字〔2019〕35号）；山西省自然科学基金项目（201901D111334）；山西省留学回国人员科技活动项目择优资助项目（20200026）；山西省回国留学人员科研资助项目（2021-142）；山西省卫健委“四个一批”科技兴医创新计划项目（2020TD05）；山西省“四个一批”科技兴医创新计划医学科技领军人才项目（2020RC05）；山西省高等学校科技创新计划项目（2019L0724）；山西中医药大学科技创新能力培育计划“基础研究专项”项目（2020PY-JC-03）；山西中医药大学青年科学家培育计划项目（2021PY-QN-03）；山西中医药大学学科建设项目（研学科函〔2021〕4号） |
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| Study on FSD-C10 Inhibiting Oxidative and Inflammatory Responses in MPTP Induced Parkinson's Disease Mice |
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FAN Huijie1, LI Yanrong1, CHAI Zhi1, LIU Jianchun1, YIN Fudong1, WANG Xinliang1, YAN Yuqing2, XIAO Baoguo3, MA Cungen1,2
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1.Neurobiology Research Center/The Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine, Shanxi University of Chinese Medicine, Jinzhong 030619, China;2.Institute of Brain Science, Shanxi Datong University, Datong 037009, China;3.Institute of Neurology, Huashan Hospital, Fudan University, Shanghai 200025, China
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| Abstract: |
| OBJECTIVE To explore the therapeutic effect and related mechanism of oral administration of FSD-C10 on Parkinson's disease(PD) mice. METHODS Mice were randomly divided into normal group, PD group and FSD-C10 group (50 mg·kg-1). The total distance, rest time and crossing numbers were evaluated by open field test. Tyrosine hydroxylase(TH) and ROCKⅡ in the brain were detected by Western blotting. Catalase(CAT), glutathione(GSH), malondialdehyde and nitric oxide(NO) were detected by colorimetric method. The levels of inflammatory factors tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β) and interleukin-6(IL-6) in the brain were measured by ELISA. RESULTS Compared with the normal group, the total distance and the crossing numbers were decreased(P<0.01), the rest time was increased(P<0.01), the level of TH was decreased(P<0.05), the level of ROCKⅡ was increased(P<0.05), the activity of CAT was decreased(P<0.05), the concentration of GSH was decreased(P<0.01), the concentration of malondialdehyde was increased (P<0.05), and the concentrations of inflammatory factors TNF-α, IL-1β, IL-6 and NO were increased(P<0.05 or P<0.01) in the PD group. Compared with the PD group, the total distance and the crossing numbers were increased(P<0.05), the rest time was decreased(P<0.01), the level of TH was increased(P<0.05), the level of ROCKⅡ was decreased(P<0.05), the activity of CAT was increased(P<0.01), the concentration of GSH was increased(P<0.01), the concentration of malondialdehyde was decreased (P<0.01), and the concentrations of inflammatory factors TNF-α, IL-1β, IL-6 and NO were decreased(P<0.01) in the FSD-C10 group. CONCLUSION Oral administration of FSD-C10 can improve the behavior of PD mice, and has a clear therapeutic effect in PD mice. The therapeutic effect may be related to the recovery of the balance of oxidation-antioxidation system and the reduction of the secretion of inflammatory factors. |
| Key words: Parkinson's disease FSD-C10 oxidative stress anti-inflammatory effect |
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