| 引用本文: | 张晨,吉飞跃,郑良凤,成守亮,王祥,郭宗锋.饱和氢盐水对短暂性脑缺血再灌注损伤小鼠海马PERK/eIF2a/ATF4信号通路的影响[J].中国现代应用药学,2021,38(7):807-813. |
| Zhang Chen,Ji Feiyue,Zheng Liangfeng,Cheng Shouliang,Wang Xiang,Guo Zongfeng.Effects of Hydrogen-rich Saline on Hippocampus PERK/eIF2α/ATF4 Signaling Pathway in Mice After Transient Cerebral Ischemia Reperfusion Injury[J].Chin J Mod Appl Pharm(中国现代应用药学),2021,38(7):807-813. |
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| 饱和氢盐水对短暂性脑缺血再灌注损伤小鼠海马PERK/eIF2a/ATF4信号通路的影响 |
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张晨1, 吉飞跃1, 郑良凤1, 成守亮1, 王祥2, 郭宗锋2
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1.南通大学附属海安医院, 中心实验室, 江苏 南通 226600;2.南通大学附属海安医院, 麻醉科, 江苏 南通 226600
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| 摘要: |
| 目的 探讨饱和氢盐水对短暂性脑缺血再灌注损伤小鼠海马PERK/eIF2a/ATF4信号通路的影响。方法 选择SPF级健康♂ C57BL/6小鼠150只，采用随机数字表法将其分为5组（n=30）：对照组、假手术组、脑缺血再灌注组、饱和氢盐水治疗组和生理盐水治疗组。于再灌注12，24 h行神经行为学评分，然后处死小鼠取海马组织，采用TUNEL法测定海马CA1区细胞凋亡指数，分别采用Western blotting和Real-time PCR法测定海马PERK、eIF2a、ATF4、CHOP和caspase-3及其mRNA的表达水平。结果 与对照组、假手术组相比，脑缺血再灌注组、饱和氢盐水治疗组和生理盐水治疗组小鼠神经行为学评分升高，海马CA1区神经细胞凋亡指数升高，海马PERK、eIF2a、ATF4、CHOP和caspase-3及其mRNA的表达上调（P均<0.05）；与脑缺血再灌注组、生理盐水治疗组相比，饱和氢盐水治疗组小鼠神经行为学评分降低，海马CA1区神经细胞凋亡指数降低，PERK、eIF2a、ATF4、CHOP和caspase-3及其mRNA的表达下调（P均<0.05）。结论 饱和氢盐水可改善小鼠短暂性脑缺血再灌注损伤，其机制可能与抑制海马PERK/eIF2a/ATF4信号通路有关。 |
| 关键词: 饱和氢盐水 脑缺血 再灌注损伤 海马 磷酸化的细胞外信号调节激酶 真核翻译起始因子2a 活化转录因子4 |
| DOI：10.13748/j.cnki.issn1007-7693.2021.07.006 |
| 分类号:R965.1 |
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| Effects of Hydrogen-rich Saline on Hippocampus PERK/eIF2α/ATF4 Signaling Pathway in Mice After Transient Cerebral Ischemia Reperfusion Injury |
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Zhang Chen1, Ji Feiyue1, Zheng Liangfeng1, Cheng Shouliang1, Wang Xiang2, Guo Zongfeng2
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1.Nantong University Hai'an Hospital, Department of Central Laboratory, Nantong 226600, China;2.Nantong University Hai'an Hospital, Department of Anesthesiology, Nantong 226600, China
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| Abstract: |
| OBJECTIVE To evaluate the effect of hydrogen-rich saline on hippocampus PERK/eIF2α/ATF4 protein signaling pathways in mice after transient cerebral ischemia reperfusion injury. METHODS One hundred and fifty healthy male C57BL/6 mice were divided into 5 groups(n=30) using a random number table: control group, sham operation group, cerebral ischemia-reperfusion group, hydrogen-rich saline treatment group and normal saline treatment group. Neurobehavioral score was assessed at 12, 24 h of reperfusion. Then mice in each group were sacrificed, and the hippocampal tissues were obtained and the apoptosis index of CA1 in hippocampus were determined by TUNEL. The expression of PERK, eIF2α, ATF4, CHOP, caspase-3 protein and mRNA were determined by Western blotting and real-time polymerase chain reaction, respectively. RESULTS Compared with the control group and the sham operation group, the neurobehavioral score and apoptosis index of hippocampal CA1 region were increased, and the expression of PERK, eIF2α, ATF4, CHOP, caspase-3 and their mRNA were up-regulated in the cerebral ischemia-reperfusion group, hydrogen-rich saline treatment group and normal saline treatment group(all P<0.05). Compared with cerebral ischemia-reperfusion group and normal saline treatment group, the neurobehavioral score and apoptosis index in hippocampus CA1 region were decreased and the expression of PERK, eIF2α, ATF4, CHOP, caspase-3 and their mRNA were down-regulated in the hydrogen-rich saline treatment group(all P<0.05). CONCLUSION Hydrogen-rich saline can improve transient cerebral ischemia reperfusion injury in mice, and its mechanism may be associated with inhibited activation of PERK/eIF2α/ATF4 signaling pathway. |
| Key words: hydrogen-rich saline brain ischemia reperfusion injury hippocampus PERK eIF2a ATF4 |
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