| 引用本文: | 胡芳,王慧娟,李俐,周英.川楝子中川楝素的不同提取工艺研究[J].中国现代应用药学,2010,27(12):1102-1106. |
| .Study on Different Extraction Methods of the Toosendanin from Fructus Toosendan[J].Chin J Mod Appl Pharm(中国现代应用药学),2010,27(12):1102-1106. |
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| 摘要: |
| 目的 对川楝子中川楝素进行提取工艺的考察。方法 采用水浴法、索氏提取和超声波细胞粉碎提取后经HPLC进行含量测定。结果 利用正交分析，得到3种提取工艺的最优条件为：①水浴法：50 ℃，80%乙醇，提取2 h，料液比为1∶30；②索氏提取法：85 ℃，70%乙醇，提取7 h，料液比为1∶20；③超声波细胞粉碎提取法：250 W，70%乙醇，提取45 min，料液比为1∶30。结论 建立的方法简便、可靠，重复性好，可为川楝子的全面研究提供一定数据参数。 |
| 关键词: 川楝子 川楝素 提取工艺 |
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| Study on Different Extraction Methods of the Toosendanin from Fructus Toosendan |
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HU Fang WANG Huijuan LI Li ZHOU Ying*
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| Abstract: |
| OBJECTIVE To study toosendanin extraction methods in Fructus toosendan. METHODS The toosendanin was extracted separately by the water bath extraction, soxhlet extraction and ultrasonic cell crushing extraction. The content of toosendanin was determinated by HPLC. RESULTS Using orthogonal experiments analysis, the optimal extraction conditions of these three extraction methods were as follows: ①The water bath extraction method: 50 ℃, 80% ethanol, extraction time 2 h, ratio of material to solvent 1∶30; ②The soxhlet extraction method: 85 ℃, 70% ethanol, extraction time 7 h, ratio of material to solvent 1∶20; ③The ultrasonic cell crushing extraction method: 250 W, 70% ethanol, extraction time 45 min, ratio of material to solvent 1∶30. CONCLUSION The established methods are simple, reliable and reproducible, which could provide some data basis in a comprehensive study of Fructus toosendan. |
| Key words: Fructus toosendan toosendanin extraction process |
