| 引用本文: | 张西如，高燕霞，姜建国，孙婷.HPLC测定氯碘羟喹的含量及有关物质[J].中国现代应用药学,2009,(10):844-846. |
| ZHANG Xiru, GAO Yanxia, JIANG Jianguo, SUN Ting.Determination of Clioquinol and Its Related Substances by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2009,(10):844-846. |
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| 摘要: |
| 目的建立氯碘羟喹的含量及有关物质的HPLC测定方法。方法采用C8柱，以乙二胺四醋酸二钠缓冲液-甲醇(35∶65)为流动相；流速1.0 mL·min-1；检测波长为254 nm。结果氯碘羟喹的线性范围为3～60 μg·mL-1(r=0.999 9)，平均回收率为99.8%，RSD为0.13% (n=9)。结论方法简便、准确，专属性强，可作为产品的含量和有关物质的检测。 |
| 关键词: 氯碘羟喹 高效液相色谱法 有关物质 |
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| Determination of Clioquinol and Its Related Substances by HPLC |
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ZHANG Xiru, GAO Yanxia, JIANG Jianguo, SUN Ting
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| Abstract: |
| OBJECTIVE To establish an HPLC method for content determination of clioquinol and related substances in clioquinol raw material. METHODS A C8 column was used with the mobile phase of the buffer solution-methanol (35∶65). The flow rate was 1.0 mL·min-1 and the detection wavelength was 254 nm. RESULTS The calibration curves of clioquinol was linear in the range of 3-60 μg·mL-1(r=0.999 9), and the average recoveries was 99.8%, RSD was 0.13%(n=9). CONCLUSION The method is rapid, accurate and reliable for determining the content and related substances in clioquinol raw material. |
| Key words: clioquinol HPLC related substances |
