| 引用本文: | 张美玉，李贻奎，张嘉，李连达.鱼腥草注射液过敏及类过敏实验研究[J].中国现代应用药学,2009,(8):610-614. |
| ZHANG Meiyu, LI Yikui, ZHANG Jia, LI Lianda.Experimental Study on Anaphylactic and Anaphylactoid Reactions of Houttuynia Cordata Injections[J].Chin J Mod Appl Pharm(中国现代应用药学),2009,(8):610-614. |
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| 摘要: |
| 目的评价鱼腥草注射液及其辅料吐温80(Tween-80)静脉给药的致敏性。方法采用Beagle犬类过敏及过敏实验，观察给药后动物行为变化和检测血浆中组胺、IgE、IgM、IgG的含量。结果含有Tween-80的鱼腥草注射液及Tween-80组动物给药后出现显著的行为异常，血浆组胺升高，而IgE变化不规律，IgG、IgM则无明显改变。结论鱼腥草注射液中导致犬严重类过敏反应与Tween-80有关。建议Beagle犬的过敏及类过敏试验，应作为中药注射液致敏试验的必做实验，行为异常及血浆中组胺为主要的判定指标，IgE作为辅助判定指标。 |
| 关键词: 鱼腥草注射液 吐温80 过敏及类过敏反应 Beagle犬 组胺 IgE |
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| Experimental Study on Anaphylactic and Anaphylactoid Reactions of Houttuynia Cordata Injections |
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ZHANG Meiyu, LI Yikui, ZHANG Jia, LI Lianda
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| Abstract: |
| OBJECTIVE To evaluate the sensitizations by intravenous of Houttuynia Cordata injections and polysorbate 80 injection. METHODS Active systemic anaphylaxis(ASA) test was carried out, by intravenous of different injections to Beagle dogs to immunize, and inject double doses of injections to stimulate in the 9 days after the last sensitized. Observe the anaphylaxis situation of Beagle dogs and measure the concentrationns of histamine, IgE, IgM, IgG in plasma with ELISA. RESULTS Polysorbate 80 injection and Houttuynia Cordata injections which include polysorbate 80 are positive on Beagle dogs after by intravenous, it's obviously behavior disorder and histamine elevation, but IgE is irregularly changed , IgM and IgG are not obvious variation. CONCLUSION Polysorbate 80 is associated with ADRS of Houttuynia Cordata Injection which leaded to severously allergic reactions. It is suggestion that anaphylactic reaction and anaphylactoid reaction tests of Beagle dogs should be carried out in the sensitization test of Chinese traditional medicine injections, behavior disorder and histamine in plasma are important assessment index, IgE is assisted one. |
| Key words: houttuynia cordata injection polysorbate 80 anaphylactic and anaphylactoid reactions beagle dogs histamine IgE |
