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| 摘要: |
| 目的建立一种灵敏、可靠的全血中加替沙星和莫西沙星的高效液相色谱荧光检测方法。方法样品经磷酸盐缓冲液提取后，用Waters Oasis MAX 小柱进行净化，甲醇-乙腈-0.2%甲酸(15∶15∶70)为流动相，采用Cloversil - C18柱（3.0 mm ×150 mm, 5 μm）分离，荧光检测波长:λex 288 nm和λem 493 nm，内标法定量。结果加替沙星和莫西沙星在15.0～240.0 μg·L-1内呈良好线性，方法回收率在97.7%～101.3% 之间，日内RSD<6.0%，其定量限为15.0 μg·L-1。结论本方法简便、灵敏、干扰少，特异性好，能满足血液中加替沙星和莫西沙星的检测要求。 |
| 关键词: 高效液相色谱-荧光法 全血 加替沙星 莫西沙星 |
| DOI： |
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| Determination of Gatifloxacin and Moxifloxacin in Whole Blood by HPLC Coupled with Fluorescence Detection |
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CHEN Xiaohong
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| Abstract: |
| OBJECTIVE To develop a sensitive and accurate method for the determination of gatifloxacin and moxifloxacin in whole blood by high-performance liquid chromatography coupled with fluorescence detection(HPLC-FLD). METHODS After gatifloxacin and moxifloxacin in whole blood were extracted by phosphate buffer, the sample was cleaned with solid-phase extraction (SPE) using Oasis MAX cartridges.The separation was performed on Cloversil - C18 column（3.0 mm ×150 mm, 5 μm）using the mobile phase consisting of methanol-acetonitrile-0.2% formic acid(15∶15∶70).Detection was carried out on a fluorescence detection at λex 288 nm and λem 493 nm. RESULTS Calibration curves were linear within the range of 15.0-240.0 μg·L-1, and the extraction recoveries were from 97.7% to 101.3%, the RSDs were less than 6.0%.The limits of quantification were found to be 15.0 μg·L-1.CONCLUSION This method is found to be simple,sensitive,little interferential and good specificity for the determination of gatifloxacin and moxifloxacin in whole blood. |
| Key words: HPLC-FLD whole blood gatifloxacin moxifloxacin |
