|
| 摘要: |
| 目的通过采用高效液相法测定河南不同产地夏枯草中熊果酸、齐墩果酸的含量来比较夏枯草质量的优劣，为夏枯草的质量控制方法提供一定的依据。方法测定熊果酸、齐墩果酸色谱条件为Betasil C18(250 mm×4.6 mm,5 μm)；流动相：磷酸二氢钠缓冲液[取磷酸二氢钠7.8 g加水至1 000 mL]-甲醇（14∶86）；流速：0.8 mL·min-1；柱温：25 ℃；检测波长：210 nm；结果熊果酸在0.554～5.34 μg内线性关系良好，r=0.999 7，平均回收率为97.45%，RSD为0.92%。齐墩果酸在0.28～2.24 μg内线性关系良好，r=0.999 4，平均回收率为97.61%，RSD为0.87%。结论该方法简单、准确并且专属性强。 |
| 关键词: 夏枯草 熊果酸 齐墩果酸 质量分析 |
| DOI： |
| 分类号: |
| 基金项目: |
|
| The Quality Analysis of Defferent Areas of Henan′s Spica Prunellae |
|
CHEN Zhihong，LIU Wei
|
| Abstract: |
| OBJECTIVE To establish an HPLC method for the determination of ursolic acid and oleanolic acid in different area of Henan′s Spica Prunellae. METHODS The ursolic acid and oleanolic acid separation was performed on a column: Betasil C18(250 mm×4.6 mm,5 μm) column, mobile phase was natrium dihydrogen phosphate solution (7.8 g→1000 mL)-methanol (14∶86), the flow rate was 0.8 mL·min-1, the column temperature was 25 ℃, the detective wavelength was 210 nm. RESULTS The ursolic acid calibration curve showed good linearity in the range of 0.554-5.34 μg(r=0.999 7), the average recovery was 97.45%(RSD=0.92%). The oleanolic acid calibration curve showed good linearity in the range of 0.28-2.24 μg (r=0.999 4), the average recovery was 97.61%(RSD=0.87%). CONCLUSION The method is simple,accurate and sensitive. |
| Key words: Spica Prunellae ursolic acid oleanolic acid quality analysis |
