双位酶免疫法测定NT-3cDNA-CHO细胞上清中rNT-3含量

季爱民;李梅;张忠义;邹恒琴

引用本文:	季爱民;李梅;张忠义;邹恒琴.双位酶免疫法测定NT-3cDNA-CHO细胞上清中rNT-3含量[J].中国现代应用药学,1999,(6):35-36.
	Ji Aimin(Ji AM),Li Mei(Li M),Zhang Zhongyi(Zhang ZY),et al.Determination of the levels of recombinant neurotrophin-3 in the supernatants of NT-3cDNA/CHO cells by two-site enzyme immunoassay[J].Chin J Mod Appl Pharm(中国现代应用药学),1999,(6):35-36.

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双位酶免疫法测定NT-3cDNA-CHO细胞上清中rNT-3含量
季爱民;李梅;张忠义;邹恒琴

作者	单位
季爱民;李梅;张忠义;邹恒琴

摘要:

关键词: 神经营养蛋白-3 抗NT-3单克隆抗体3W3 双位酶免疫测定法(EIA)

DOI：

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基金项目:广东省自然科学基金!资助项目（９６０３６４）

Determination of the levels of recombinant neurotrophin-3 in the supernatants of NT-3cDNA/CHO cells by two-site enzyme immunoassay

Ji Aimin(Ji AM),Li Mei(Li M),Zhang Zhongyi(Zhang ZY),et al

Abstract:

OBJECTIVE:To determine the levels of recombinant neurotrophin-3(NT-3) in the supernatants of NT-cDNA/CHO cells.METHOD:A two-site enzyme immunoassay(EIA) method was established using the monoclonal antibody 3W3.RESULTS:The limit of detection of the EIA was

Key words: neurotrophin 3(NT-3),anti NT-3 monoclonal antibody 3W3,two site enzyme immunoassay(EIA)