基于聚乙烯亚胺的非病毒基因载体研究进展

苗佩宏; 何彩霞; 高建青; 胡忠杰<sup>*</sup>

引用本文:	苗佩宏,何彩霞,高建青,胡忠杰.基于聚乙烯亚胺的非病毒基因载体研究进展[J].中国现代应用药学,2011,28(6):505-510.
	MIAO Peihong,HE Caixia,GAO Jianqing,HU Zhongjie.Progress of PEI-based Cationic Polymers as Non-viral Gene Vectors[J].Chin J Mod Appl Pharm(中国现代应用药学),2011,28(6):505-510.

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基于聚乙烯亚胺的非病毒基因载体研究进展
苗佩宏¹, 何彩霞², 高建青², 胡忠杰¹
1.武警浙江省总队医院药械科，嘉兴 314000;2.浙江大学药物制剂研究所，杭州 310058

摘要:

如今，越来越多的非病毒基因载体被应用于基因传递中，聚乙烯亚胺(PEI)作为一种重要的阳离子聚合非病毒基因传递载体受到广泛的关注。针对如何克服其生物不可降解性和细胞毒性并进一步提高转染效率的问题，笔者通过文献综合介绍了一些新的策略，并具体对以PEI为基础的新型载体的合成、新型体外转染方法的建立以及体内应用等方面的研究进展进行综述。

关键词: 非病毒载体基因传递聚阳离子复合物聚乙烯亚胺疏水化修饰反向转染三维转染

DOI：

分类号:

基金项目:国家自然科学基金(30873173，30973648)；浙江省自然科学基金杰出青年团队项目(R2090176)；中央高校基本科研业务费专项资金

Progress of PEI-based Cationic Polymers as Non-viral Gene Vectors

MIAO Peihong¹, HE Caixia², GAO Jianqing², HU Zhongjie¹

1.Department of Pharmacy, Zhejiang Provincial Corps Hospital of Chinese People's Armed Police Forces, Jiaxing 314000, China;2.Zhejiang University, Institute of Pharmaceutics, Hangzhou 310058, China

Abstract:

An increasing number of non-viral vectors are being developed for the use of gene delivery nowadays. As a kind of versatile vector, poly(ethylene-imine) (PEI)-based cationic polymers receives intensive attention. Most of these research efforts are focused on its non-biodegradablity and cytotoxicity while several strategies have been explored to enhance the gene transfection efficiency of PEI-based derivatives. In this review, some of the recent development of PEI-based gene delivery vector, including synthesis of novel PEI-based vectors, construction of new PEI-based transfection system in vitro as well as in vivo applications were summarized.

Key words: non-viral vector gene delivery cationic polymer poly (ethylene-imine) hydrophobic modification reverse transfection three-dimensional transfection