Abstract: OBJECTIVE To study and reveal the spectrum activity relationship and efficacy material foundation of Panax notoginseng on protection of rat myocardial ischemia. METHODS Established Panax notoginseng HPLC fingerprint analytical method and then used the method to research the spectrum activity relationship and efficacy material foundation of Panax notoginseng. RESULTS The main effective components were ginseng saponin Rg₁, Rb₁ and notoginseng saponins R₁. CONCLUSION Through the research on the relationship between the spectrum and efficiency, we directly obtained efficacy active substance and established the new method to evaluate Panax notoginseng medicine spectrum activity relationship. It objectively reflectes the inherent quality of the drugs and provides a new strategy for the further research of traditional Chinese medicine.