Abstract: OBJECTIVE To synthesize four new types of contrast media. METHODS 5-amino-2,4,6-triiodoisophthaloyl chloride was used as starting material with a variety of processes including alkylation, acetylation or chloroacetylation and hydrolization to synthesize the crude product. Then the crude product was purified by ion exchange resin column chromatography and ODS chromatography to purify the title product. RESULTS The structure of the product was identified by MS and NMR, and the imaging effect was compared to iohexol and ioversol. CONCLUSION The imaging effect of four novel contrast media was significant and contrast effect is comparable with commercial products.