Abstract: OBJECTIVE To study the clinical value of laparoscopic operation combined with Caulis Sargentodoxae Formula on endometriosis. METHODS Ninety cases diagnosed endometriosis who were underwent laparoscopic operations were divided into three groups at random, 30 cases were treated with Caulis Sargentodoxae Formula as the Caulis Sargentodoxae Formula group, 30 cases were treated with mifepristone as the mifepristone group, the other 30 cases as the control group. RESULTS The total effective rate was significantly higher in the Caulis Sargentodoxae Formula group and in the mifepristone group than that of in the control group (P<0.05). After treatment, serum MMP-9 level was significant lower and serum TIMP-1 level was significant higher in the Caulis Sargentodoxae Formula group and in the mifepristone group than that of in the control group. The ratio of MMP-9/TIMP-1 was also significant lower in the Caulis Sargentodoxae Formula group and in the mifepristone group than that of in the control group(P<0.05). CONCLUSION Caulis Sargentodoxae Formula can significantly relieve the symptoms and signs of patients with endometriosis and improve inner environment of pelvic cavity by regulate the ratio of MMP-9 /TIMP-1.