HPLC测定维药恰玛古儿药材中槲皮素的含量

Content Determination of Quercetin in Brassica Rapa L. by HPLC

摘要: 目的建立维药恰玛古儿药材中槲皮素的含量测定方法，为其制定质量标准提供依据。方法采用HPLC对恰玛古儿药材中槲皮素进行含量测定。采用Kromasil C₁₈柱(4.6 mm×250 mm，5μm)，流动相：甲醇-0.1%磷酸水溶液(50∶50)，检测波长：360 nm，流速：1.0 mL·min^-1，柱温：30 ℃。结果槲皮素进样量在2.525 6~15.153 6 μg·mL^-1时，与峰面积值呈良好线性关系(r=0.999 7)。平均加样回收率为101.4％，RSD为1.1％(n=6)。结论所建立的方法简便、快捷，重复性好，可用于该药材的质量评价。

Abstract: OBJECTIVE To establish the content determination method of quercetin in Brassica rapa L., and so as to provide reference for its quality control. METHODS The content of quercetin in Brassica rapa L. was determined by HPLC. The chromatographic conditions were as follows: Kromasil C₁₈(4.6 mm×250 mm, 5 μm), mobile phase was methanol-0.1% phosphoric acid(50∶50), flow rate 1.0 mL·min^-1, column temperature at 30 ℃, detection wavelength 360 nm. RESULTS Querectin showed a good linear relationship in the range of 2.525 6-15.153 6 μg·mL^-1, the average recovery was 101.4% and RSD was 1.1%. CONCLUSION The established method is simple and rapid with good reproducibility, and can be used for the quality control of Brassica rapa L.