HPLC测定六味地黄浓缩丸中5-HMF的含量

Determination of 5-Hydroxymethylfurfural in Liuweidihuang Pills by HPLC

摘要: 目的建立六味地黄浓缩丸中5-羟甲基糠醛(5-HMF)的含量测定方法。方法采用Agilent Zorbax C₁₈色谱柱 (250 mm×4. 6 mm，5 μm)；流动相为甲醇-水(20∶80)；流速：1.0 mL·min^-1；检测波长：280 nm；柱温：30 ℃。结果 5-HMF在0.026 2~2.62 mg·mL^-1内线性关系良好，平均回收率为99.7%，RSD为0.57%。结论该方法简便、快速、准确度好，可有效控制该制剂中5-羟甲基糠醛含量。

Abstract: OBJECTIVE To establish an HPLC method for the determination of 5-hydroxymethylfurfural(5-HMF) in Liuweidihuang pills. METHODS The HPLC method was performed on an Agilent Zorbax C₁₈ column(250 mm×4.6 mm, 5 μm) with 284 nm as the detection wavelength, and methanol-water(20∶80) was used as mobile phase. The column temperature was 30 ℃, and the flow rate was 1.0 mL·min^-1. RESULTS The regression results which was based on weighted least square method showed a good linearity within the range of 0.026 2-2.62 mg·mL^-1, and the average recovery was 99.7% with RSD of 0.57%. CONCLUSION The HPLC method was accurate, sensitive and suitable for determining the content of 5-HMF in Liuweidihuang pills.