Abstract: OBJECTIVE To establish a method for microbial limit test of cefuroxime axetil capsule.METHODS Based on the viable count of test bacteria to choice method and identify the most sensitive strains，by using low-speed centrifugal precipitation test solution and membrane filtration to remove the antibacterial activity of cefuroxime axetil.It was confirmed whether its antibacterial activity had been removed thoroughly. RESULTS The method passed the validation test of Ch.P(2005).The Bacillus subtilis and Staphylococcus aureus were the most sensitive strain.CONCLUSION This method can be an effective method for the quality control of the medicines, and can be used in microbial limit test for cefuroxime axetil capsule.